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Key Documents

A268

Sigma-Aldrich

Anti-A1 Adenosine Receptor antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

peso molecular

antigen 36-40 kDa

reatividade de espécies

human, rat, bovine (weak)

técnica(s)

immunohistochemistry (frozen sections): 1:100
immunoprecipitation (IP): suitable
western blot: 1:1,000

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... ADORA1(134)
rat ... Adora1(29290)

Descrição geral

ADORA1 is an adenosine receptor which is part of the G-protein coupled receptor family of proteins. ADORA1 functions as a receptor for adenosine to regulate vasodilation and vasoconstriction in cardiac tissue and kidneys.
Adenosine receptors (ARs) are members of the 7-transmembrane domain G protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of Ars ubiquitously mediates cell signalling pathways that protect tissues and organs from damage . Additionally, Ars modulate immune functions of the brain and alters the neurotransmission by glutamatergic and dopaminergic systems
The A1AR is a glycoprotein of MW 36-40 kDa that can activate Gi and Go proteins in vitro In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of K+ channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system
A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release In white adipocytes, A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis
Anti-A1 Adenosine Receptor is specific for A1 adenosine receptor adenosine receptor subunit. By immunoblotting, it reacts strongly with human and rat, and weakly with bovine A1. The antibody detects A1 adenosine receptor in human hippocampus by immunohistology and may be used for immunoprecipitation and confocal microscopy

Imunogênio

synthetic peptide (Gln-Pro-Lys-Pro-Pro-Ile-Asp-Glu-Asp-Leu-Pro-Glu-Glu-Lys-Ala-Lys-Ala-Glu-Asp) derived from amino acids 309-326 of the rat A1 adenosine receptor C-terminal domain.

Aplicação

Rabbit anti-A1 adenosine receptor antibody can be used for immunohistochemistry and immunoblotting assays using rat tissues. The antibody can also be used for immunocytochemistry assays using rat spinal cord tissues. The product may also be used for immunoprecipitation applications.

forma física

Solution in phosphate buffered saline containing 1 mg/mL BSA and 0.05% sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Christopher S Rex et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 25(25), 5956-5966 (2005-06-25)
Memory loss in humans begins early in adult life and progresses thereafter. It is not known whether these losses reflect the failure of cellular processes that encode memory or disturbances in events that retrieve it. Here, we report that impairments
S A Rivkees et al.
Brain research, 677(2), 193-203 (1995-04-24)
Polyclonal antisera were generated against two identical regions of rat and human A1 adenosine receptors using synthetic multiple-antigenic-peptides as immunogens. Western blotting showed that the antisera recognized a single protein in brain of the expected size for A1 receptors. Immunohistochemistry
Stefania Gessi et al.
Advances in pharmacology (San Diego, Calif.), 61, 41-75 (2011-05-19)
The adenosine receptors A(1), A(2A), A(2B), and A(3) are important and ubiquitous mediators of cellular signaling, which play vital roles in protecting tissues and organs from damage. In particular, adenosine triggers tissue protection and repair by different receptor-mediated mechanisms, including
Role of adenosine A1 and A3 receptors in regulation of cardiomyocyte homeostasis after mitochondrial respiratory chain injury
Schneyvays V et al
American Journal of Physiology. Heart and Circulatory Physiology, 288, H2792-H2792 (2004)
M Schindler et al.
Neuroscience letters, 297(3), 211-215 (2001-01-04)
Adenosine exerts its physiological actions by binding to G-protein coupled receptors, four of which have been identified and cloned to date (A1, A2a, A2b and A3). Here we report the development of anti-human adenosine A1, receptor anti-peptide polyclonal antibodies and

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