AB1589P
Anti-Adenosine A2b Receptor Antibody
Chemicon®, from rabbit
Sinônimo(s):
A2b Receptor Antibody, Adenosine A2b Antibody, Anti-Adenosine Receptor
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
affinity purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
purificado por
affinity chromatography
reatividade de espécies
rat, human, mouse
fabricante/nome comercial
Chemicon®
técnica(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... ADORA2B(136)
Especificidade
Adenosine A2b receptor. Recognizes a band of approximately 50-52 kDa in several rat, mouse and human tissues (thymus, placenta, colon and small intestine). The antibody also recognizes the 35 kDa band in small intestine. No bands were detected in skeletal muscle or heart.
Imunogênio
Amino acid sequence corresponding to the 2nd extracellular domain (16 amino acids) of the human brain A2bR gene (Pierce et al. 1992; Tucker & Linden 1993).
Aplicação
Detect Adenosine A2b Receptor using this Anti-Adenosine A2b Receptor Antibody validated for use in ELISA, IC, IH & WB.
Immunohistochemistry: 10-50 μg/mL on frozen and formalin fixed tissue sections. The receptor was localized in epithelial cells of the crypts, and in synctiotrophoplast cells of human placenta and in T84 human colon adenocarcinoma cells.
Western blot: 1-10 μg/mL (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.
ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG292)/well)
Optimal working dilutions must be determined by end user.
Western blot: 1-10 μg/mL (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.
ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG292)/well)
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Circadian Rhythm & Sleep
Neurotransmitters & Receptors
Circadian Rhythm & Sleep
forma física
Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.
Armazenamento e estabilidade
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Characterization of adenosine receptors in bovine corneal endothelium.
Kah Y Tan-Allen, Xing Cai Sun, Joseph A Bonanno
Experimental Eye Research null
Adenosine stimulates fibroblast growth factor-7 gene expression via adenosine A2b receptor signaling in dermal papilla cells.
Iino, M; Ehama, R; Nakazawa, Y; Iwabuchi, T; Ogo, M; Tajima, M; Arase, S
The Journal of Investigative Dermatology null
Diabetes-induced alterations of adenosine receptors expression level in rat liver.
Marzena Grden,Marzena Podgorska,Andrzej Szutowicz,Tadeusz Pawelczyk
Experimental and Molecular Pathology null
A2BR adenosine receptor modulates sweet taste in circumvallate taste buds.
Kataoka, S; Baquero, A; Yang, D; Shultz, N; Vandenbeuch, A; Ravid, K; Kinnamon, SC; Finger, TE
Testing null
A short cerebral ischemic preconditioning up-regulates adenosine receptors in the hippocampal CA1 region of rats.
Ai-Min Zhou, Wen-Bin Li, Qing-Jun Li, Hui-Qing Liu, Rong-Fang Feng, Hong-Gang Zhao
Neuroscience Research null
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