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Sigma-Aldrich

DCFHDA

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Sinônimo(s):

2′,7′-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein

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About This Item

Fórmula empírica (Notação de Hill):
C24H14Cl2O7
Número CAS:
2044-85-1
Peso molecular:
485.27
Beilstein:
68488
Número MDL:
MFCD00037501
Código UNSPSC:
12352108
ID de substância PubChem:
57649438
NACRES:
NA.32

linha de produto

BioReagent

Ensaio

≥95% (HPLC)

pf

232-234 °C (lit.)

solubilidade

DMSO: soluble

fluorescência

λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

adequação

suitable for fluorescence

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3

chave InChI

VQVUBYASAICPFU-UHFFFAOYSA-N

Descrição geral

2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Aplicação

2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells.

Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Gloves

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