14324C

Meio sem soro EX-CELL^® 302 para células de CHO

without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• Número MDL: MFCD01634638
• Código UNSPSC: 12352207
• NACRES: NA.75

Propriedades

• descrição: for research or for further manufacturing use
• esterilidade: sterile-filtered
• forma: liquid
• técnica(s): cell culture | mammalian: suitable
• componentes: L-glutamine: no; NaHCO₃: 1.60 g/L; HEPES: 7.5 mM; glucose: 3.42 g/L; phenol red: yes
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Aplicação

EX-CELL 302 is a serum-free medium which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR^- or Glutamine Synthetase, or the GS System^™, because it does not contain hypoxanthine, thymidine or L-glutamine. For use with the DHFR^- selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kDa. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Lutrol has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.

Atenção

Não congelar. Manter ao abrigo da luz. Diversos componentes do meio são instáveis sob a luz e não devem ser expostos a luz por longos períodos.

Informações legais

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

GS System is a trademark of Lonza Group Ltd.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable