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Key Documents

12106C

Sigma-Aldrich

Soro bovino fetal

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture

Sinônimo(s):

FBS, FCS, soro, soros

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.71

fonte biológica

bovine fetus

Nível de qualidade

esterilidade

sterile-filtered

linha de produto

FBS Premier

composição

hemoglobin, ≤20 mg/dL

origem

USA origin

técnica(s)

cell culture | mammalian: suitable

Impurezas

≤10 EU/mL endotoxin

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

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Aplicação

Fetal Bovine Serum (FBS) provides many non-defined growth promoting and survival enhancing factors to cells in culture. FBS, heat inactivated may be used in place of regular FBS wherein the inactivation of the complement system is beneficial to cell growth or function. Heat-inactivated FBS is used to culture Leishmania sp.

Nota de preparo

Prepared by heating at 56°C for 30 minutes.
Prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes is used to inactivate the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Nota de análise

  • Testado quanto à presença de endotoxinas e hemoglobina
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves


Certificados de análise (COA)

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Protocolos

WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.

Protocol Guide: XTT Assay for Cell Viability and Proliferation

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