08124
Isoamylase from Pseudomonas sp.
ammonium sulfate suspension, ≥10,000,000 units/mg protein
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About This Item
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
Produtos recomendados
Formulário
ammonium sulfate suspension
atividade específica
≥10,000,000 units/mg protein
temperatura de armazenamento
2-8°C
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Aplicação
Isoamylase from Pseudomonas sp. has been used in starch debranching and to measure the chain-length distributions.
Ações bioquímicas/fisiológicas
Pseudomonas amyloderamosa is considered a popular source of isoamylase. In the presence of a surfactant, this isoamylase produces a series of maltooligosaccharides. Immobilized isoamylase makes it applicable with a polysaccharide matrix composed of agarose, cellulose, and raw corn starch.
Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Definição da unidade
One unit causes an increase in A610 of 0.1 in 1 hour using a homogenized solution of rice starch as a substrate (pH 3.5, 40°C)
Nota de análise
≥10 MU/mg protein
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2
Código de classe de armazenamento
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Starch debranching is fundamental for understanding the structure-function relationships of starch. In this paper, atomic force microscopy (AFM) was used to investigate potato starch by isoamylase [EC 3.2.1.68] debranching at nanometer scale. The hydrolysates were separated by gel-permeation chromatography and
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