RSTREP-RO

Streptavidin, recombinant

liquid, ≥17 units/mg protein, pH range 5.0-9.0

• Sinônimo(s): streptavidin
• Código UNSPSC: 12352202

Propriedades

• fonte biológica: Streptomyces sp. ( Streptomyces avidinii)
• Nível de qualidade: 100
• recombinante: expressed in E. coli
• forma: liquid
• atividade específica: ≥17 units/mg protein
• peso molecular: ~52 kDa
• embalagem: pkg of 1 mg (11721666001); pkg of 5 mg (11721674001)
• fabricante/nome comercial: Roche
• concentração: 70-90%
• técnica(s): protein array: suitable; protein purification: suitable
• Impurezas: 6-7% water
• faixa de pH: 5.0-9.0
• pI: 7.4-7.7 (two main bands)
• solubilidade: water: 10 mg/L (mA₄₀₅=100)
• adequação: suitable for immunological tests
• nº de adesão UniProt: P22629
• Condições de expedição: cooler
• temperatura de armazenamento: -10 to -25°C

Descrição

Descrição geral

Streptavidin, a highly versatile protein, is naturally produced by Streptomyces avidinii. It exhibits a strong affinity for biotin, forming a non-covalent yet robust binding interaction. This unique property makes streptavidin an ideal choice for various biological detection systems, including western blotting, enzyme-linked immunosorbent assays (ELISA), and immunohistochemistry.

Aplicação

Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).

Sequência

Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.

Definição da unidade

One unit binds 1 μg D-biotin at pH 8.9.

forma física

White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solution
Protein: 0.7 ± 0.1mg protein lyophilizate (A₂₈₂ = 3.1)
K_D: 10^-15M

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash