11214667001
Roche
Anti-Digoxigenin, Fab fragments
from sheep
Sinônimo(s):
anti-digoxigenin, digoxigenin
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About This Item
Código UNSPSC:
12352203
Produtos recomendados
fonte biológica
sheep
Nível de qualidade
conjugado
unconjugated
forma do anticorpo
affinity purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
Formulário
lyophilized
embalagem
pkg of 1 mg
fabricante/nome comercial
Roche
técnica(s)
ELISA: 2-4 μg/mL
immunohistochemistry: 0.5-1 μg/mL
western blot: 0.5-2 μg/mL
Isotipo
IgG1
temperatura de armazenamento
2-8°C
Descrição geral
Anti-Digoxigenin, Fab fragments are Fab fragments from polyclonal anti-digoxigenin antibodies. Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems.
Especificidade
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Aplicação
Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.
Use Anti-Digoxigenin, Fab fragments for the detection of digoxigenin-labeled compounds using:
Use Anti-Digoxigenin, Fab fragments for the detection of digoxigenin-labeled compounds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
- Flow cell construction.
Características e benefícios
Specific to digoxigenin and digoxin.
Sequência
The number of amino groups in the Fab fragments is not known, because polyclonal antibodies are not sequenced. But as a rule of thumb full length antibodies contain about 80 amino groups on average.
Nota de preparo
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
Storage conditions (working solution): Diluted antibody is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!
- ELISA: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 1 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Storage conditions (working solution): Diluted antibody is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!
Reconstituição
Add 1 ml PBS to a final concentration of 1 mg/ml. Centrifuge reconstituted antibody for 5 minutes at 10,000 rpm prior to each use, and pipet the necessary amount carefully from the surface.
Nota de análise
Preparation: After immunization with digoxigenin, sheep IgGs are purified by ion exchange chromatography. Specific IgGs are isolated by immunosorption. Fab fragments obtained after papain digestion are purified by gel filtration and lyophilized.
Outras notas
For life science research only. Not for use in diagnostic procedures.
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Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
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Artigos
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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