11333054001

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester

>90% (elementary analysis), pkg of 5 mg, powder, pH range 2-13

• Sinônimo(s): Digoxigenin NHS-ester, dig-nhs-ester, ε-(Digoxigenin-3-0-acetamido)caproic acid N-hydroxysuccinimide ester, DIG NHS
• Fórmula empírica (Notação de Hill): C₃₅H₅₀N₂O₁₀
• Número CAS: 129273-26-3
• Peso molecular: 658.78
• Número MDL: MFCD02093394
• Código UNSPSC: 41105600
• ID de substância PubChem: 329749387

Propriedades

• Nível de qualidade: 100
• Ensaio: >90% (elementary analysis)
• forma: powder
• peso molecular: M_r 658.8
• embalagem: pkg of 5 mg
• fabricante/nome comercial: Roche
• faixa de pH: 2-13
• solubilidade: DMF: soluble; DMSO: soluble; ethanol: soluble
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: [H][C@]12CC[C@]3([H])[C@]([H])(C[C@@H](O)[C@]4(C)[C@H](CC[C@]34O)C5=CC(=O)OC5)[C@@]1(C)CC[C@@H](C2)OCC(=O)NCCCCCC(=O)ON6C(=O)CCC6=O
• InChI: 1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35+/m1/s1
• chave InChI: KHNDABJZSPPYLE-FUGFVFQCSA-N

Descrição

Descrição geral

Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH₂-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.

Aplicação

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester has been used to label the sequence (III) for the linker molecule sequence (VIII).

Ações bioquímicas/fisiológicas

Digoxigenin, a non-radioactive immune-tag is mainly used for labeling proteins and in situ hybridization.

Princípio

The activated ester reacts with amino groups under mild conditions. The digoxigenin moiety can be introduced into proteins or 5′-amino substituted oligonucleotides.

Nota de preparo

Working concentration: 1 to 5 mM
Working solution: Preparation of working solution

Under stirring 0.327 mg digoxigenin-3-O-methylcarbonyl- ε-aminocaproic acid -N-hydroxysuccinimide ester is dissolved in 8.18 μl DMSO or ethanol.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Pictogramas: GHS06,GHS08,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H300,H319,H331,H373,H410
• Declarações de precaução: P260 - P264 - P273 - P301 + P310 + P330 - P391 - P403 + P233
• Classificações de perigo: Acute Tox. 2 Oral - Acute Tox. 3 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - STOT RE 2
• Código de classe de armazenamento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable