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Documentos Principais

11175041910

Roche

DIG Nucleic Acid Detection Kit

greener alternative

sufficient for 40 blots (10 cm x 10 cm each), kit of 1 (5 components), suitable for hybridization, suitable for Northern blotting

Sinônimo(s):

DIG system

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About This Item

Código UNSPSC:
41116134

uso

sufficient for 40 blots (10 cm x 10 cm each)

Nível de qualidade

embalagem

kit of 1 (5 components)

fabricante/nome comercial

Roche

características do produto alternativo mais ecológico

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

técnica(s)

Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable

categoria alternativa mais ecológica

temperatura de armazenamento

−20°C

Descrição geral

DIG Nucleic Acid Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP (alkaline phosphatase) antibody conjugate and the color substrates NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate).
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Aplicação

DIG nucleic acid detection kit has been used in the confirmation of genetic manipulation.
The DIG Nucleic Acid Detection Kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:
  • Southern blots
  • Northern blots
  • Other nucleic acid blotting applications
  • In situ hybridization applications
The kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:
  • Southern blots
  • Northern blots
  • Other nucleic acid blotting applications
  • In situ hybridization applications

Características e benefícios

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Embalagem

1 kit containing 5 components.

Princípio

DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP antibody conjugate and the color substrates NBT and BCIP.

Nota de preparo

Working concentration: Flow cytometry: 2 μg/ml (0.2 μg/100 μl/106 cells); Immunohistocytochemistry: 6 μg/ml
Working concentration of conjugate depends on application and substrate.
Storage conditions (working solution):
  • Anti-Digoxigenin-AP Conjugate (vial 3): 2 to 8 °C, it is stable for 12 months at this temperature
  • NBT/BCIP (vial 4): 2 to 8 °C, stable
    Note: During shipment of the kit on dry ice, a precipitate may occur which is dissolved by briefly warming to 37 °C
  • The blocking reagent (vial 5) is stable for 36 months and can be stored dry at 4 to 8 °C.
  • Autoclaved stock solution can be stored for several days to a week either unopened at 15 to 25 °C or at 4 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.

Somente componentes do kit

Nº do produto
Descrição

  • DIG-labeled Control DNA 5 µg/ml

  • DNA Dilution Buffer, [50 μg/ml] fish sperm DNA

  • Anti-Digoxigenin-AP Conjugate antibody 750 U/ml

  • NBT/BCIP, 50x stock solution 50x concentrated

  • Blocking Reagent

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

does not flashNot applicable

Ponto de fulgor (°C)

does not flashNot applicable


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Artigos

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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