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11333062910

Roche

Anti-Digoxigenin

from mouse IgG1κ (clone 1.71.256)

Sinônimo(s):

anti-digoxigenin, digoxigenin

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About This Item

Código UNSPSC:
12352203

fonte biológica

mouse

Nível de qualidade

100

conjugado

unconjugated

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

clone 1.71.256, monoclonal

forma

lyophilized

embalagem

pkg of 100 μg

fabricante/nome comercial

Roche

técnica(s)

ELISA: 2-4 μg/mL
immunocytochemistry: 0.5-2 μg/mL
immunohistochemistry: 0.5-2 μg/mL
western blot: 0.5-2 μg/mL

Isotipo

IgG1κ

temperatura de armazenamento

2-8°C

Descrição geral

Contents: Lyophilizate
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.

Especificidade

The monoclonal antibody reacts with free and bound digoxigenin.

Aplicação

Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot
  • Immunofluorescence staining
  • FISH (fluorescent in situ hybridization)

Ações bioquímicas/fisiológicas

In the presence of Na+, Mg2+ and adenosine triphosphate (ATP), digoxigenin inhibits sodium pumps.

Características e benefícios

  • Specific to free and bound digoxigenin
  • The antibody is not stabilized with protein and therefore suitable as coating antibody in Sandwich-ELISAs and for labeling procedures. For example it can be conjugated with enzymes or fluorescence dyes for direct detection of digoxigenin-labeled samples.

Nota de preparo

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: for coating: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 2 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications
phosphate buffered saline, pH 7.4

Reconstituição

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.

Armazenamento e estabilidade

The lyophilized antibody is stable at +2 to +8°C. The reconstituted antibody solution is stable up to 6 months at +2 to +8°C. The solution can be stored in aliquots at -15 to -25°C. Avoid repeated freezing and thawing.

Nota de análise

There are no cross reactivities known.

Outras notas

For life science research only. Not for use in diagnostic procedures.

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Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H317

Classificações de perigo

Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Nonradioactive labeling of probe with digoxigenin by polymerase chain reaction
<BIG>Lion T and Haas OA</BIG>
Analytical Chemistry, 188, 335-337 (1990)
Magda Rudzka et al.
The Plant cell, 34(6), 2404-2423 (2022-03-17)
Gene regulation ensures that the appropriate genes are expressed at the proper time. Nuclear retention of incompletely spliced or mature mRNAs is emerging as a novel, previously underappreciated layer of posttranscriptional regulation. Studies on this phenomenon indicated that it exerts
Carla Winterling et al.
RNA biology, 11(1), 66-75 (2014-01-21)
A growing body of evidence suggests the non-protein coding human genome is of vital importance for human cell function. Besides small RNAs, the diverse class of long non-coding RNAs (lncRNAs) recently came into focus. However, their relevance for infection, a
Affinity Labeling of a Sulfhydryl Group in the Cardiacglycoside Receptor Site of Na+/K+-ATPase by N-Hydroxysuccinimidyl Derivatives of Digoxigenin
<BIG>Antolovic R, et al.</BIG>
European Journal of Biochemistry, 227, 61-67 (1995)
Xingqi Chen et al.
BioTechniques, 56(3), 117-118 (2014-03-20)
Current techniques for analyzing chromatin structures are hampered by either poor resolution at the individual cell level or the need for a large number of cells to obtain higher resolution. This is a major problem as it hampers our understanding
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