10107921001
Roche
Nuclease S7
Micrococcal nuclease, from Staphylococcus aureus
Sinônimo(s):
s7 nuclease
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About This Item
Código UNSPSC:
12352204
Produtos recomendados
fonte biológica
Staphylococcus aureus
Nível de qualidade
forma
lyophilized
atividade específica
~15,000 U/mg
embalagem
pkg of 15,000 U
fabricante/nome comercial
Roche
pH ideal
7.5
Condições de expedição
wet ice
Aplicação
- Removal of endogenous RNA from in vitro translation systems
- RNA sequencing experiments
Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.
Ações bioquímicas/fisiológicas
Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca2+ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.
Definição da unidade
One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A260.
Armazenamento e estabilidade
Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.
Outras notas
For life science research only. Not for use in diagnostic procedures.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Órgãos-alvo
Respiratory system
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
Certificados de análise (COA)
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