MABE1114

Anti-CENP-C Antibody, clone AL61A

clone AL61A, from mouse

• Sinônimo(s): Centromere protein C, CENP-C, CENP-C 1, Centromere autoantigen C, Centromere protein C 1, Interphase centromere complex protein 7
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clone: AL61A, monoclonal
• application: ICC; IP
• reatividade de espécies: human
• técnica(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: AL61A, monoclonal
• reatividade de espécies: human
• técnica(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_001803
• nº de adesão UniProt: Q03188
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CENPC(1060)

Descrição

Descrição geral

Centromere protein C (UniProt Q03188; also known as CENP-C, CENP-C 1, Centromere autoantigen C, Centromere protein C 1, Interphase centromere complex protein 7) is encoded by the CENPC (also known as CENPC1, ICEN7) gene (Gene ID 1060) in human. Kinetochores assemble on centromeres to bind spindle microtubules and mediate chromosome segregation during cell division. CENP-C is a subunit of constitutive centromere-associated network (CCAN) that acts as the centromere-kinetochore interface. CCAN is a large complex composed of at least 16 different centromeric proteins (CENPs). CENP-C plays an important role in kinetochore assembly downstream of CENP-A. The PEST domain in the N-terminal half of CENP-C interacts directly with the CCAN subcomplex CENP-HIKM (CENP-H, CENP-K, CENP-I, and CENP-M) and CENP-C depletion is shown to casue mislocalization of CENP-T and the CENP-HIKM complex subunits in HeLa cells.

Especificidade

Expected to react with both spliced isoforms of human CENP-C reported by UniProt (Q03188).

Imunogênio

Epitope: N-terminal.

GST-tagged recombinant human CENP-C N-terminal fragment.

Aplicação

Anti-CENP-C Antibody, clone AL61A is an antibody against CENP-C for use in Immunocytochemistry, Immunoprecipitation.

Immunoprecipitation Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant immunoprecipitated CENP-C from HeLa nuclear extract (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).
Immunocytochemistry Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant detected nuclear CENP-C puncta by fluorescent immunocytochemistry staining of permeabilized HeLa and Jurkat cells (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).
Immunocytochemistry Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant immunostained CENP-C-transfected, but not ZBTB32-transfected, HEK293T cells (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).

Qualidade

Evaluated by Immunocytochemistry in Jurkat cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected CENP-C in Jurkat cells.

Descrição-alvo

106.8 kDa (isoform 1) and 61.54 kDa (isoform 2) calculated.

forma física

Format: Purified

Outras notas

Concentration: Please refer to lot specific datasheet.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable