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MABE1069

Sigma-Aldrich

Anti-BAP1 Antibody, clone 3C11

clone 3C11, from mouse

Sinônimo(s):

Ubiquitin carboxyl-terminal hydrolase BAP1, EC 3.4.19.12, BRCA1-associated protein 1, Cerebral protein 6

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

3C11, monoclonal

reatividade de espécies

human

técnica(s)

immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1κ

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

ambient

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... BAP1(8314)

Descrição geral

Ubiquitin carboxyl-terminal hydrolase BAP1 (EC 3.4.19.12; UniProt Q92560; also known as BRCA1-associated protein 1, Cerebral protein 6) is encoded by the BAP1 (also known as KIAA0272, hucep-6) gene (Gene ID 8314) in human. Originally identified and named as a BRCA1-interacting protein, BAP1 is a tumor suppressor that participates in DNA-damage response, DNA synthesis and cell cycle progression. BAP1 belongs to the ubiquitin C-terminal hydrolase (UCH) family of deubiquitin enzymes (DUBs), all members of which, including UCH-L1, UCH-L3 and UCH-L5, are characterized by a conserved UCH catalytic domain (CD; BAP1 a.a. 1-235). BAP1 and UCH-L5 also share a conserved C-terminal ULD domain (a.a. 640-710 of BAP1). The region between BAP1 CD and ULD is important for binding to the HCF-1 protein. In addition, BAP1 contains a C-terminal extension (CTE; a.a. 711-729) following its ULD. Study shows that while CTE tethers BAP1 to the nucleosome core, additional interaction with ASXL1 DEUBiquitinase ADaptor domain (DEUBAD) is needed to allow BAP1 substrate processing by increasing it′s affinity towards ubiquitin. In a similar fashion, UCH-L5 activity is reugulated via interaction with DEUBAD of RPN13 and INO80G. BAP1 is implicated in transcription regulation due to its interaction with transcription-related proteins, such as FOXK2, HCF-1, OGT, LSD2, MBD-6, MBD-5, ASXL1 and ASXL2. BAP1 loss leads to a variety of cancers, including metastatic cutaneous and uveal melanoma, pleural mesothelioma, renal cell carcinoma and bladder tumours.

Especificidade

Clone 3C11 specifically detects BAP1 in human. It targets an epitope within amino acids 337-440 in human BAP1.

Imunogênio

GST-tagged full length recombinant human ubiquitin carboxyl-terminal hydrolase BAP1.

Aplicação

Anti-BAP1 Antibody, clone 3C11, Cat. No. MABE1069, is a highly specific mouse monoclonal antibody that targets BAP1 and has been tested in and Immunoprecipitation and Western blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: 10 µg/mL from a representative lot detected BAP1 in whole cell extracts of HEK293T cells over-expressing BAP1 protein. (Courtesy of Hongzhuang Peng, Ph.D. and Frank J. Rauscher III, Ph.D., The Wistar Institute, Philadelphia PA USA).

Western Blotting Analysis: A representative lot detected BAP1 in Western Blotting applications (Abdel-Wahab, O., et. al. (2012). Cancer Cell. 22(2):180-93).

Immunoprecipitation Analysis: A representative lot detected BAP1 in Immunoprecipitation applications (Abdel-Wahab, O., et. al. (2012). Cancer Cell. 22(2):180-93).

Qualidade

Evaluated by Western Blotting in HEK293T cells overexpressing BAP1.

Western Blotting Analysis: A 1:125 dilution of this antibody detected BAP1 in 10 µg of lysate from HEK293T cells overexpressing BAP1.


Descrição-alvo

~ 80 kDa observed; 80.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1


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