ABN1739M

Anti-Utrophin A

from rabbit

• Sinônimo(s): UTRN A
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: human, mouse
• embalagem: antibody small pack of 25 μg
• técnica(s): immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG
• nº de adesão NCBI: NP_035812
• nº de adesão UniProt: E9Q6R7
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... UTRN(7402)

Descrição

Descrição geral

Utrophin A (UniProt: E9Q6R7) is encoded by the Utrn gene in murine species. Utrophin is a large cytoskeletal protein, which is autosomally-encoded homologue of dystrophin, the protein product of the Duchenne muscular dystrophy (DMD) gene. It displays a sequence homology with dystrophin and possesses many of the protein-binding properties ascribed to dystrophin. In normal skeletal muscle, utrophin is located at the neuromuscular junction and dystrophin predominates at the sarcolemma. During development and in certain myopathies utrophin is also reported to be present at the sarcolemma and this redistribution is associated with higher levels of utrophin. Utrophin is found to co-localize with the acetylcholine receptors at the neuromuscular junctions and may participate in stabilizing the synaptic cytoskeleton. The utrophin mRNA contains two full-length species (named A- and B-utrophin), which are transcribed from different promoters. Hence, utrophin is a composite of A- and B-utrophin and only the A-utrophin is up-regulated in dystrophin-deficient striated muscle. This up-regulation is reported to occur post-transcriptionally. Utrophin A and B differ in their N-termini sequence. (Ref.: Blake, DJ et al. (1996). Brain Pathol. 6(1); 37-47; Weir, AP et al. (2002). J. Biol. Chem. 277 (47); 45285-90).

Especificidade

This rabbit polyclonal antibody detects utrophin in human and mouse muscle tissue. It targets an epitope with in the first 26 amino acids from the N-terminal region.

Imunogênio

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the first 26 amino acids from the N-terminal region of murine utrophin.

Aplicação

Anti-Utrophin A Antibody, Cat. No. ABN1739, is a highly specific rabbit polyclonal antibody that targets Utrophin and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Utrophin A in human skeletal muscle and human uterus tissue.

Immunofluorescence Analysis: A representative lot detected Utrophin A in quadriceps transverse sections in transgenic mice (Adams, M.E., et. al. (2010). J Neurosci. 30(33):11004-10).

Immunohistochemistry Analysis: A representative lot detected Utrophin A in frozen sections of the gastrocnemius muscles (Banks, G.B., et. al. (2014). PLoS Genet. 10(6):e1004431).

Western Blotting Analysis: representative lot detected Utrophin A in whole muscle lysates (Banks, G.B., et. al. (2014). PLoS Genet. 10(6):e1004431).

Western Blotting Analysis: A representative lot detected Utrophin A in muscle membrane extracts from mdx mice (Courtesy of Dr Marvin Adams at University of Washington).

Immunofluorescence Analysis: A representative lot detected Utrophin A in mouse neuromuscular junction (Courtesy of Dr Marvin Adams at University of Washington).

Research Category
Neuroscience

Qualidade

Evaluated by Immunohistochemistry in human prostate.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Utrophin A in human prostate tissue.

Descrição-alvo

394.47 kDa calculated.

forma física

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash