MAB6020

Anti-Cytochrome P450 11β-Hydroxylase Antibody, clone 11B2-1A4

culture supernatant, clone 11B2-1A4, Chemicon^®

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: culture supernatant
• tipo de produto de anticorpo: primary antibodies
• clone: 11B2-1A4, monoclonal
• reatividade de espécies: rat
• fabricante/nome comercial: Chemicon^®
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1
• nº de adesão UniProt: P30099
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: rat ... Cyp11B1(500892)

Descrição

Especificidade

Rat p450 11beta-Hydroxylase

Imunogênio

Aa 272-285 (KNVYRELAE GRQQS-C)

Aplicação

Anti-Cytochrome P450 11β-Hydroxylase Antibody, clone 11B2-1A4 is an antibody against Cytochrome P450 11β-Hydroxylase for use in IH(P) & WB.

Research Category
Metabolism

Research Sub Category
Enzymes & Biochemistry

Western blot: 1:300

Immunohistochemistry (S.T.F. on paraffin embedded tissue required, traditional formalin fixation or paraformaldehyde fixation is not recommended. Microwave or pressure cooker antigen retrieval; blocking with a solution of 0.5% SDS, 2% milk and 5% NGS in Tris-HCL pH 7.6. Primary antibody is diluted in PBS or TBS typically with no SDS or NGS: 1:50-200, usually overnight refrigerated. After washing 4X with Tris buffer pH 7.6, secondary goat anti-mouse secondary (AP124B) is incubated (1:500-1:2000) diluted in a solution of 2% milk, 5% NGS, Tris-HCL pH 7.6 and 0.05% tween 20. Detection is via streptavidin HRP/DAB. Recommended counter stain is Gill′s Haematoxylin.

Optimal working dilutions must be determined by end user.

STF fixative is available from streck laboratories, www.streck.com).

forma física

Supernatant. Liquid in 0.1% sodium azide.

Armazenamento e estabilidade

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable