MAB1285F

Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated

clone HLA.ABC.m3, Chemicon^®, from mouse

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• conjugado: FITC conjugate
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: HLA.ABC.m3, monoclonal
• reatividade de espécies: human
• fabricante/nome comercial: Chemicon^®
• técnica(s): flow cytometry: suitable; immunofluorescence: suitable
• Isotipo: IgG2a
• nº de adesão NCBI: NM_005514.5
• nº de adesão UniProt: P01889
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... HLA-B(3106)

Descrição

Descrição geral

BIOCHEMISTRY: pI = 8.0

The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.

Especificidade

This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7

(9.5x107M-1).

Imunogênio

Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal Protein A.

Aplicação

Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated detects level of HLA-B27 & has been published & validated for use in FC & IF.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.

SUGGESTED USAGE DILUTION

Flow cytometry and immunofluorescence 1:15 to 1:60

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

forma física

Purified from mouse ascitic fluid. Fluorescein conjugated. The antibody is supplied in 0.5ml phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.

Armazenamento e estabilidade

Store at 2 to 8°C. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable