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Sigma-Aldrich

Rabbit Anti-Sheep IgG Antibody, HRP conjugate

Chemicon®, from rabbit

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.46

fonte biológica

rabbit

Nível de qualidade

conjugado

peroxidase conjugate

forma do anticorpo

affinity purified immunoglobulin

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

reatividade de espécies

sheep

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
western blot: suitable

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Descrição geral

Affinity purified rabbit anti-sheep IgG, Peroxidase conjugated
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Aplicação

EIA and Western blots: 1:5,000-1:100,000.

Immunohistochemistry: 1:500-1:5,000.

Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
This Rabbit anti-Sheep IgG Antibody, HRP conjugate is validated for use in ELISA, WB for the detection of Sheep IgG.

forma física

ImmunoAffinity Purified
RECONSTITUTION:

Reconstitute with 1.5 mL of sterile distilled water.

Armazenamento e estabilidade

STORAGE: Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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