AB9566-I

Anti-OTX2 Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): OTX2, Homeobox protein OTX2, Orthodenticle homolog 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): rhesus macaque (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• técnica(s): immunohistochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NP_758840
• nº de adesão UniProt: P32243
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... OTX2(5015)

Descrição

Descrição geral

Homeobox protein OTX2 (UniProt P32243; also known as Orthodenticle homolog 2) is encoded by the OTX2 (also known as CPHD6, MCOPS5) gene (Gene ID 5015) in human. OTX2 is a paired-type homeobox transcription factor that controls the expression of target genes by binding to regulatory regions containing the YTAATNN motifs. Studies conducted in mice shows developmental stage-dependent expression pattern. OTX2 is widely expressed in the epiblast, but becomes progressively regionalised during gastrulation to an anterior domain of the ectoderm (anterior neuroectoderm, ANE) that contains the precursor cells of the prosencephalon and the mesencephalon of the embryonic brain. The precursor cells for the anterior visceral endoderm (AVE) fail to translocate from the distal site to the anterior region of the pre-gastrulation stage Otx2-knockout embryos. The absence of the AVE, in turn, causes the loss of the source of morphogenetic signals for inducing neural differentiation of the ANE. In addtion, OTX2 is shown to be essential in the anterior mesendoderm (AME) for the induction of anterior neural tissues and head formation. OTX2 is reported to mediate the transcriptions of Dkk1 and Lhx1, loss of Otx2 in the AME results in the downregulation of Dkk1 and Lhx1.

Especificidade

Expected to react with both spliced isoforms, as well as all five polymorphic forms of human OTX2 reported by UniProt (P32243). The immunogen sequence is about 78% conserved in OTX1, this polyclonal antibody may therefore recognize OTX1. However, OTX1 cross-reactivity has not been directly tested and confirmed. OTX2 is expressed earlier than OTX1 and therefore this antibody can be used to identify OTX2 during development.

Imunogênio

Epitope: Internal region

KLH-conjugated linear peptide corresponding to the internal region of human OTX2.

Aplicação

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected OTX2 in human retina tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Qualidade

Evaluated by Western Blotting in Y79 human retinoblastoma cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected OTX2 in 10 µg of Y79 human retinoblastoma cell lysate.

Descrição-alvo

~35 kDa observed

forma física

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1