SAB4200543

Anti-Calbindin-D-28K antibody, Mouse monoclonal

clone CB-955, purified from hybridoma cell culture

• Sinônimo(s): Monoclonal Anti-(28kD), Monoclonal Anti-D-28K, Monoclonal Anti-D-dependent calcium-binding protein, Monoclonal Anti-RTVL-H protein, Monoclonal Anti-avian-type, Monoclonal Anti-calbindin 1, Monoclonal Anti-vitamin
• Código UNSPSC: 12352203
• NACRES: NA.44

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: CB-955, monoclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~28 kDa
• reatividade de espécies: mouse, human, rat
• validação aprimorada: independent; Learn more about Antibody Enhanced Validation
• concentração: ~1.0 mg/mL
• técnica(s): immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin embedded rat cerebellum.; western blot: 2-4 μg/mL using MDBK extract.
• Isotipo: IgG1
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CALB1(793)

Descrição

Descrição geral

Anti-Calbindin-D-28K antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CB-955 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified bovine kidney calbindin-D-28K.

Calbindin-D-28K (CALB1) is encoded by the gene mapped to human chromosome 8q21.3-q22.1. CALB1 belongs to the EF hand superfamily of Ca²⁺ binding proteins (CBPs). The encoded protein contains EF hand motif involved in the specific and reversible binding of Ca^2+. It is highly expressed in specific regions of central nervous system such as cerebellar Purkinje cells and hippocampal granule cells.

Imunogênio

purified bovine kidney calbindin-D-28K. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.

Aplicação

• Anti-Calbindin-D-28K antibody, Mouse monoclonal has been used in:
• western blotting
• enzyme-linked immunosorbent assay (ELISA){31
• immunohistochemistry
• immunohistochemical procedure
• indirect immunofluorescence

Ações bioquímicas/fisiológicas

Calbindin D-28k might play a vital role as an intracellular Ca²⁺ buffer. It has an ability to protect nerve cells from calcium-mediated neurotoxicity. The encoded protein functions as a potent neuroanatomical marker which can be used to selectively visualize certain neurons and pathways in the central nervous system and peripheral nervous system. Mutations in the gene has been observed in patients with Parkinson′s disease (PD).

Calbindin-D (28K) functions as a mobile or partly immobilized Ca²⁺ -buffer with medium kinetics and affinity buffer function. Second, it functions in buffered Ca²⁺- diffusion-transport function. Finally, it interacts with target proteins in its apo-and Ca²⁺-loaded form-sensor- like function. Over-expression of this protein stimulates neurite outgrowth in dopaminergic neuronal cells.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable