AB1928
Anti-Integrin α5 Antibody, CT, Intracellular
serum, Chemicon®
Sinônimo(s):
CD49e, Fibronectin Receptor alpha Subunit
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
serum
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
reatividade de espécies
human, chicken, hamster, rat, mouse
fabricante/nome comercial
Chemicon®
técnica(s)
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... ITGA5(3678)
Descrição geral
Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.
Especificidade
Antibody recognizes integrin alpha5. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. No cross-reactivity to alpha1, alpha2, alpha3, alpha4, alpha6 or alphaV. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus, intracellular.
Reactivity with other species has not been determined.
Imunogênio
Synthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha5 integrin subunit.
Aplicação
Anti-Integrin α5 Antibody, C-terminus, Intracellular detects level of Integrin α5 & has been published & validated for use in ELISA, IC, IF, IH, IP, RIA & WB.
Western Blot Analysis:
1:1000 dilution of a previous lot was used.
ELISA:
1:500-1:2000 dilution of a previous lot was used in ELISA.
Immunoprecipitation:
Recommended use of 5 μL of antibody for 5x106 cells.
Immunohistochemistry:
1:1000 dilution of a previous lot was used in tissue staining; suggested for use on acetone fixed tissue only.
Immunocytochemistry:
1:1000 of a previous lot was used in immunocytochemistry.
Optimal working dilutions must be determined by the end user.
1:1000 dilution of a previous lot was used.
ELISA:
1:500-1:2000 dilution of a previous lot was used in ELISA.
Immunoprecipitation:
Recommended use of 5 μL of antibody for 5x106 cells.
Immunohistochemistry:
1:1000 dilution of a previous lot was used in tissue staining; suggested for use on acetone fixed tissue only.
Immunocytochemistry:
1:1000 of a previous lot was used in immunocytochemistry.
Optimal working dilutions must be determined by the end user.
Qualidade
Routinely evaluated by Western Blot on HUVEC lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected integrin alpha 5 on 10 μg of HUVEC lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected integrin alpha 5 on 10 μg of HUVEC lysates.
Descrição-alvo
114 kDa
forma física
Rabbit polyclonal antiserum in buffer containing 0.05% sodium azide.
Nota de análise
Control
Mouse 3T3 fibroblasts Skin (Basement Membrane).
Mouse 3T3 fibroblasts Skin (Basement Membrane).
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Preços
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Placenta null
D L Livant et al.
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Using naturally serum-free SU-ECM basement membranes as invasion substrates showed that plasma fibronectin was necessary to stimulate invasion by DU 145 human and metastatic MATLyLu (MLL) rat prostate carcinoma cells. This activity mapped to the PHSRN sequence, which induced invasion
S Khan et al.
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Animal Reproduction Science, 80(1-2), 91-100 (2004)
D Chun et al.
Neuroscience, 105(4), 815-829 (2001-09-01)
Three structurally distinct groups of antagonists were used to test the hypothesis that integrin adhesion receptors play an essential role in consolidating (stabilizing) long term potentiation of the Schaffer collaterals in rat hippocampus. Comparisons were made of percent potentiation at
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