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Key Documents

06-340

Sigma-Aldrich

Anti-Myc Antibody

Upstate®, from rabbit

Sinônimo(s):

Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

reatividade de espécies

human, mouse, Xenopus, avian

fabricante/nome comercial

Upstate®

técnica(s)

ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

Informações sobre genes

human ... MYC(4609)

Descrição geral

c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression.

Especificidade

Recognizes Myc isoforms Mr 50-67kDa.
Will also cross-react with avian and Xenopus.

Imunogênio

Bacterially expressed fusion-protein corresponding to the full-length human Myc.

Aplicação

Immunoprecipitation:
4 µg of a previous antibody immuno-precipitated Myc as shown by an independent laboratory.

Immunocytochemistry:
This antibody has been used for immunocytochemistry by an independent laboratory. (Ohtsuka, T., 1998)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-Myc Antibody (rabbit polyclonal antibody) validated in ChIP, ICC, IP, WB to detect Myc also known as Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein.

Qualidade

Routinely evaluated by western blot on A431 lysates from EGF-stimulated human A431 cells, human A431 cells and HeLa nuclear extract.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected Myc in A431 lysates from EGF-stimulated human A431 cells. 0.5-2 µg/mL of a previous lot detected Myc in RIPA lysates from human A431 cells and HeLa nuclear extract.

Descrição-alvo

67 kDa

forma física

Format: Purified
Immunoaffinity Chromatography
Purified rabbit polyclonal IgG in buffer containing PBS, 0.05% sodium azide with 2 mg/mL BSA. Frozen solution.

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

Expression of c-Myc in response to colony-stimulating factor-1 requires mitogen-activated protein kinase kinase-1.
M Cheng, D Wang, M F Roussel
The Journal of Biological Chemistry null
Sex hormone-binding globulin selectively modulates estradiol-regulated genes in MCF-7 cells.
M G Catalano,L Costantino,R Frairia,G Boccuzzi,N Fortunati
Hormone and Metabolic Research null
Loss of JunB activity enhances stromelysin 1 expression in a model of the epithelial-to-mesenchymal transition of mouse skin tumors.
Hulboy, DL; Matrisian, LM; Crawford, HC
Molecular and cellular biology null
T-cell lymphomas mask slower developing B-lymphoid and myeloid tumours in transgenic mice with broad haemopoietic expression of MYC.
Smith, DP; Bath, ML; Harris, AW; Cory, S
Oncogene null
Polyamine depletion up-regulates c-Myc expression, yet induces G(1) arrest and terminal differentiation of F9 teratocarcinoma stem cells.
L Frostesjo, O Heby, L Frostesjo, O Heby
Journal of Cellular Biochemistry null

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