05-117

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1

clone bFM-1, Upstate^®, from mouse

• Sinônimo(s): Basic fibroblast growth factor, basic fibroblast growth factor bFGF, fibroblast growth factor 2, fibroblast growth factor 2 (basic), heparin-binding growth factor 2., GSK3 beta, GSK3B, Glycogen synthase kinase 3 beta, Glycogen synthase kinase 3β
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: bFM-1, monoclonal
• Formulário: liquid
• peso molecular: 17.5 kDa
• reatividade de espécies: human, mouse, bovine, rat
• fabricante/nome comercial: Upstate^®
• técnica(s): neutralization: suitable; radioimmunoassay: suitable
• Isotipo: IgG1κ
• adequação: not suitable for Western blot
• nº de adesão NCBI: NM_002006.3
• nº de adesão UniProt: P09038
• Condições de expedição: dry ice
• Informações sobre genes: human ... FGF2(2247) ; mouse ... Fgf2(281161) , Fgf2(14173) ; rat ... Fgf2(54250)

Descrição

Descrição geral

Fibroblast growth factor 2 (FGF-2) belongs to the family of heparin-binding growth factors. It is localized in the cells of the mesoderm and neuroectodermal origin and several tumor cells. FGF-2 exists in four isoforms. It is made up of four cysteine residues, many basic residues, and two active sites. FGF-2 protein is a trigonal pyramidal structure formed by twelve anti-parallel β-sheets. The FGF-2 chromosome is located on the human chromosome at 4q28.1.

Research area: Cancer

Especificidade

This antibody is highly specific for the non-denatured form of FGF-2/basic FGF from bovine, human, rat and mouse sources. Does not cross-react with bovine acidic FGF.

This antibody recognizes the non-denatured form of FGF-2/basic FGF; does not cross-react with bovine acidic FGF.

Imunogênio

Epitope: a.a. 5-24

Purified bovine brain basic FGF (HBGF-2). Clone bFM-1.

Aplicação

Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 has been used in:

• FGF2 neutralization assay (6 or 12 µg/mL)
• in vitro tube formation assay (1000 ng/mL)
• immunolabelling (1:200)

Ações bioquímicas/fisiológicas

Fibroblast growth factor 2 (FGF-2) plays a role in vertebrate embryonic and fetal development. It acts as an inducer of angiogenesis and is a well-known mitogen (in vitro) for a variety of cells including fibroblasts and vascular endothelial cells. FGF-2 exerts its biological activity through high-affinity tyrosine kinase receptors and low-affinity heparan sulfate proteoglycans present on the cell surface. It exhibits autocrine effects on various cell functions involved in angiogenesis such as proliferation, integrin expression, migration, and proteinase production. FGF-2 participates in the growth, migration, differentiation, and survival of a variety of cells. The binding of FGF-2 with heparin protects FGF-2 from denaturation by heat and acid and proteolytic cleavage.

Qualidade

Routinely evaluated by neutralization of greater than 50% of growth of FBHE cells simulated with FGF-2/bFGF.

Neutralization of bFGF Activity Assay:
2.5 µg/mL of this lot of antibody neutralized greater than 40% of growth of FBHE cells simulated with 5 ng/mL of FGF-2/bFGF.

Descrição-alvo

17.5 kDa

forma física

Ammonium sulfate precipitation and DEAE-cellulose chromatography

Format: Purified

Purified mouse monoclonal IgG1κ in TBS. Protein determined by a Bradford microtiter assay using mouse IgG as the standard. Frozen solution.

Armazenamento e estabilidade

Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análise

Control
Fetal liver or kidney tissue.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable