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UHPLC (Isocratic) Analysis of 14 Cannabinoids on Ascentis® Express C18

UHPLC (Isocratic) Analysis of 14 Cannabinoids on Ascentis® Express C18 application for UHPLC

Materials

analytical column

Product No.
Description
Pricing

53825-U

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

accessory

Product No.
Description
Pricing

51163-U

EXP® Pre-Column Filter

holder with EXP titanium hybrid ferrule, pack of 1 sets

51164-U

EXP® Pre-Column Filter Cartridges

0.5μm, pkg of 5 ea

standard

Product No.
Description
Pricing

C-045

Cannabidiol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-046

Cannabinol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

T-005

(−)-trans-Δ9-THC solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

T-032

(−)-Δ8-THC solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-140

Cannabidivarin solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-141

Cannabigerol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-142

Cannabigerolic acid solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

C-143

Cannabichromene solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-144

Cannabidiolic acid solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

T-094

Tetrahydrocannabivarin (THCV) solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

T-093

Delta9-Tetrahydrocannabinolic acid A solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

C-152

Cannabidivarinic Acid (CBDVA) solution

1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®

C-150

Cannabichromenic Acid (CBCA) solution

1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®

T-111

Tetrahydrocannabivarinic acid (THCVA) solution

1.0 mg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®

CONDITIONS

column

Ascentis Express C18, 15 cm x 2.1 mm, 2.7 μm particles (53825-U)

mobile phase

[A] 0.1% formic acid in HPLC water; [B] 0.1% formic acid in acetonitrile; (30:70, A:B)

flow rate

0.6 mL/min

pressure

6390 psi (440 bar)

column temp.

20 °C

detector

UV, 230 nm

injection

2 μL

sample

25 μg/mL each compound in acetonitrile

Description

Analysis Note

Cannabinoids are a group of active compounds found in cannabis. They reportedly provide relief to an assortment of symptoms in the treatment of pain, nausea, anxiety, mood disorders, and inflammatory diseases. Cannabinoids imitate naturally occurring compounds and interact with two specific types of receptors: CB1 and CB2, located in the nervous system and immune system, respectively. Shown here is an isocratic separation of fourteen cannabinoids in less than twelve minutes on an Ascentis® Express C18 column. Cerilliant cannabinoid CRMs provide reliable identification and quantification. These standards are used in testing methods by GC/MS, LC/MS, or HPLC for applications in clinical toxicology, testing of cannabis potency or impurity profiling by growers, pharmaceutical research, forensic analysis, and urine drug testing.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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