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Solid Phase Micro-Extraction (SPME)

Assembled SPME fiber with a nitinol-core

Solid phase microextraction (SPME) is a versatile and solvent-free sample preparation technique. It works on the principle of adsorption/absorption and desorption and uses a fiber coated with an extractive phase to concentrate analytes in a sample. It successfully combines the sampling, isolation, and enrichment of an analyte to one step. SPME is used widely for the sampling of environmental volatiles, food & beverages, flavors & fragrances, forensics & toxicology, and pharmaceutical samples.    

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Smart SPME fibers

The smart SPME fibers are an extension of our expertise and innovation from traditional SPME coatings to smart SPME format for accurate, reliable, and consistent results. Our smart fibers including Carboxen® fibers, dual coated fibers, or overcoated fibers are fitted with a unique chip to offer seamless sample preparation and other including benefits:

  • Traceability to check usage, stroke count, dates of operation, and temperature exposure
  • High-throughput sample preparation
  • Automatic application of correct parameters for each fiber coating

See for more information on Smart SPME Fibers for Automated Sampling.

nitinol-core SPME fibers

The nitinol-core of SPME fibers enhances the fiber performance and the precision of chromatographic results. Fibers with either Carboxen®/Polydimethylsiloxane (CAR/PDMS) or Polydimethylsiloxane/ Divinylbenzene (PDMS/DVB) coatings are available with a nitinol-core. Other benefits of nitinol fibers include:

  • Enhanced lot-to-lot and fiber-to-fiber reproducibility
  • Higher fiber durability
  • Similar selectivity and inertness compared to fused silica core fibers

See for more information on Nitinol Core SPME Fibers.

OVERCOATED (OC) SPME FIBERS

The over coating of fibers is a new technology in fiber coating to protect the SPME fiber from matrix components. This allows for immersion of the fiber directly into complex samples ensuring a more efficient extraction (e.g. of semivolatile compounds). Polydimethylsiloxane (PDMS) is used to overcoat a PDMS/divinylbenzene (DVB) fiber. The resulting fiber is more physically robust and resistant to chemical fouling and allows for an efficient wash step to also remove adhering matrix.

See Overcoated Fibers available.

BioSPME

BioSPME technology greatly improves upon the speed and simplicity compared with current bioanalytical sample preparation techniques employed for plasma protein binding studies and other free analyte analyses. The primary benefits of Supel™ BioSPME devices include:

  • 3x faster workflow compared to rapid equilibrium dialysis based methods
  • Universal 96-pin format, compatible with standard consumables
  • Simple workflow amenable to total automation or manual operation

See for more information on Supel™ BioSPME 96-Pin Devices.

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GC Columns, Accessories & Adsorbents
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Solid Phase Extraction (SPE) and QuEChERS
Discover diverse materials for solid phase extraction (SPE) and QuEChERS sample prep. Cartridges, tubes, plates, and more available.

Related Applications
Solid Phase Microextraction (SPME)
Solid phase microextraction (SPME) is a solvent-free sample preparation technique that uses coated fibers to extract analytes of interest from a sample before analysis by GC or HPLC.
Sample Preparation by Filtration
Filtration separates substances based on physical or chemical properties. Lab samples are routinely filtered before analysis to reduce sample complexity and increase analyte purity. This allows scientists to generate higher quality analytical results.
Sample Purification
A range of purification methods may be used to prepare a sample prior to downstream analysis. Purification reduces sample complexity, removes interferences, and concentrates an analyte before analysis.
QuEChERS Sample Preparation Method
QuEChERS is a ‘Quick, Easy, Cheap, Effective, Rugged and Safe’ sample cleanup method used to prepare food and agricultural samples for pesticides analysis. QuEChERS is based on the Solid Phase Extraction (SPE) technique of sample preparation.
Solid Phase Extraction (SPE)
Solid phase extraction (SPE) is a technique used for rapid, selective sample preparation and purification prior to chromatographic analysis (e.g. HPLC, GC, TLC). SPE is used to exchange sample matrices, concentrate analytes, and remove interferences and contaminants to improve data and protect the analytical column.
Gas Chromatography (GC)
Gas chromatography is a common analytic technique used to separate and analyze volatile compounds in the gas phase. GC is applied in many industries for quality control, and to identify and/or quantify compounds in a mixture.
Mass Spectrometry
Mass spectrometry (MS) is an analytical tool used to identify compounds, determine chemical structure, and assess isotopic abundance. In MS, samples are ionized, and the resulting ions are identified based on their mass-to-charge (m/z) ratios.

Related Services
Custom SPME Fibers
Solid phase micro extraction is a solvent-free microextraction technique for a broad range of samples and matrix. To ensure accurate and reliable testing results to meet your analytical needs, we offer custom SPME fiber configurations. These customizations include features such as phase chemistry, film thickness, needle gauge and length, and fiber core material.

Highlights


Webinar: Complete Workflow Solutions
for Analyzing Complex Food Samples
using SPME

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