Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

P9172

Sigma-Aldrich

Pyronin Y

for NA electrophoresis

Synonym(s):

Pyronin Y, Pyronin G, Pyronin J

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C17H19ClN2O
CAS Number:
92-32-0
Molecular Weight:
302.80
Colour Index Number:
45005
Beilstein/REAXYS Number:
3795789
EC Number:
202-147-8
MDL number:
MFCD00011725
UNSPSC Code:
12171500
PubChem Substance ID:
57654569
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

form

powder

composition

Dye content, ≥45%

technique(s)

nucleic acid detection: suitable

mp

250-260 °C (lit.)

solubility

water: 10 mg/mL, dark red

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

Application

Pyronin Y has been used:
  • in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
  • as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
  • staining of CD34+ cells

Biochem/physiol Actions

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Preparation Note

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Green, F.J., ed.
The Sigma-Aldrich Handbook of Stains, Dyes and Indicators,, 601-602 (1990)
A gel electrophoresis method for determining the relative binding constants of biologically active, intercalating fluorescent stains.
S M Hassur et al.
Analytical biochemistry, 95(2), 329-339 (1979-06-01)
Senescence and apoptosis block hematopoietic activation of quiescent hematopoietic stem cells with short telomeres
Wang J, et al.
Blood, 124(22), 3237-3240 (2014)
Photosensitizing effects of the tricyclic heteroaromatic cationic dyes pyronin Y and toluidine blue O (tolonium chloride
Darzynkiewicz Z and Carter SP
Cancer research, 48(5) (1988)
Staining of RNA after polyacrylamide gel electrophoresis.
K Marcinka
Methods in cell biology, 10, 49-68 (1975-01-01)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service