A6014

Acridine Orange hemi(zinc chloride) salt

For nucleic acid staining in cells or gels

• Synonym(s): 3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt, 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt), Basic Orange 14
• Linear Formula: C₁₇H₂₀ClN₃ · HCl · 1/2ZnCl₂
• CAS Number: 10127-02-3
• Molecular Weight: 369.96
• Colour Index Number: 46005
• Beilstein/REAXYS Number: 3734978
• EC Number: 233-353-6
• MDL number: MFCD00081043
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891048
• NACRES: NA.52

Properties

• Quality Level: 200
• product line: BioReagent
• form: powder
• composition: Dye content, ~80%
• technique(s): nucleic acid detection: suitable
• solubility: ethanol: 2 mg/mL; 4 mg/mL (2-methoxyethanol (EGME)); water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)
• suitability: suitable for flow cytometry; suitable for microscopy
• storage temp.: room temp
• SMILES string: Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C
• InChI: 1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
• InChI key: RAHGLSRJKRXOSY-UHFFFAOYSA-L

Description

General description

Acridine orange is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.

Application

Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

Suitable for

• detection of nucleic acids separated by gel electrophoresis
• fluorescence and epifluorescence microscopy
• analysis of mitochondria and lysosomes by flow cytometry
• DNA staining in apoptosis studies

Features and Benefits

• 120 microM of acridine orange detects 25-50 ng of purified DNA per band in gels
• differential staining of single- and double-stranded polynucleotides

Principle

Acridine orange intercalates into the nucleic acids of double helix and is detectable as green fluorescence at 530 nm. It binds electrostatically to the phosphate groups in single stranded nucleic acids and is detectable at red fluorescence at 640 nm.

Safety Information

• pictograms: GHS08
• signalword: Warning
• hcodes: H341
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)