158550

Acridine Orange hemi(zinc chloride) salt

Dye content 85 %

• Synonym(s): 3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt, 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt), Basic Orange 14
• Linear Formula: C₁₇H₂₀ClN₃ · HCl · 1/2ZnCl₂
• CAS Number: 10127-02-3
• Molecular Weight: 369.96
• Colour Index Number: 46005
• Beilstein/REAXYS Number: 3734978
• EC Number: 233-353-6
• MDL number: MFCD00081043
• UNSPSC Code: 12171500
• PubChem Substance ID: 24849765
• NACRES: NA.47

Properties

• form: powder
• Quality Level: 200
• composition: Dye content, 85%
• solubility: H₂O: 1 mg/mL, clear, orange to very deep orange
• λmax: 489 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C
• InChI: 1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
• InChI key: RAHGLSRJKRXOSY-UHFFFAOYSA-L

Description

Application

Acridine Orange hemi (zinc chloride) salt has been used to stain primary human umbilical vein endothelial cells (HUVEC) and is used in micronucleus tests (MNTs).

Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

Biochem/physiol Actions

Acridine orange (AO) is a fluorescent dye with two different staining characteristics. In fixed tissues, it acts as a metachromatic dye and differentially stains DNA (green) and RNA (orange). But, in living cells it acts as a pH indicator and produce brilliant orange-red in acidic condition. AO has been used to identify apoptotic cells in drosophila embryos. Acridine Orange staining method has been used to deliver more consistent data in the micronucleus test.

Safety Information

• pictograms: GHS08
• signalword: Warning
• hcodes: H341
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)