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T8154

Sigma-Aldrich

Trypan Blue solution

0.4%, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
Blue cell viability dye
Empirical Formula (Hill Notation):
C34H24N6Na4O14S4
CAS Number:
Molecular Weight:
960.81
Beilstein:
4360496
MDL number:
PubChem Substance ID:
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

form

liquid

storage condition

dry at room temperature

concentration

0.4%

technique(s)

cell culture | mammalian: suitable
tissue processing: suitable

application(s)

cell analysis

shipped in

ambient

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

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General description

Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.
Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.

Application

Trypan Blue solution has been used:
  • in cell viability assay to count viable and dead cells
  • in rescue assay to count viable cells
  • in trypan blue exclusion counting method to determine proliferation curve
  • as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
  • in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
  • to count GEC cells using a hemocytometer

Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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