MilliporeSigma
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302643

Sigma-Aldrich

Trypan Blue

Dye content 60 %

Synonym(s):
Direct blue 14
Linear Formula:
C34H24N6O14S4Na4
CAS Number:
Molecular Weight:
960.81
Colour Index Number:
23850
Beilstein:
4360496
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

form

powder

composition

Dye content, 60%

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

λmax

607 nm

ε (extinction coefficient)

≥60000 at 603-607 nm in methanol at 0.01 g/L
60000 at 603-607 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Gene Information

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Application

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Packaging

25, 100 g in glass bottle

Biochem/physiol Actions

Trypan blue is a blue acid dye that contains two azo chromophores. It is a large, hydrophilic, tetrasulfonated anionic dye. It is also used to screen the cytotoxic properties of plant extracts. In addition, trypan blue is used for staining epiretinal membranes during vitrectomy surgery.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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