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Evans Blue

Dye content ≥75 %

Direct Blue 53
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
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PubChem Substance ID:



Quality Level


Dye content, ≥75%


diagnostic assay manufacturing

storage temp.

room temp

SMILES string




InChI key


Gene Information

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Evans blue has been used to check cell viability. It has been used to study BBB permeability.


10, 50, 100 g in glass bottle

Biochem/physiol Actions

Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability. It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.


Health hazard

Signal Word


Hazard Statements

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should Product E2129, Evans Blue, be dissolved?

    This product is soluble in water, alcohol, acids and alkalies.

  4. What is the solution stability of Product E2129, Evans Blue?

    This product is rather stable in aqueous solution and may be autoclaved at 15 psi for 30 min. However, dye made up in physiological saline should not be autoclaved.

  5. What is the difference between Evans Blue Products E2129 and 206334?

    Both products are Evans Blue. Product No. E2129 has a dye content of 80% and Product No. 206334 has a dye content of approximately 85%.

  6. What is the shelf life of Product E2129, Evans Blue?

    This product has a retest date of 4 years.  This information can be found on the Certificate of Analysis.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Can Evans Blue, Product E2129, be used as a counterstain for FITC?

    This product can be used as a counterstain in immunohistochemistry when using FITC. After staining for immunofluorescence, dip sections in a 0.1% (w/v) in water solution of Evans Blue for 5-10 minutes. Rinse well in fresh PBS or water before coverslipping. Reference: Immunocytochemistry, Theory and Practice, p. 82 (1988).

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Pilhan Kim et al.
Nature methods, 7(4), 303-305 (2010-03-17)
In vivo imaging of small animals offers several possibilities for studying normal and disease biology, but visualizing organs with single-cell resolution is challenging. We describe rotational side-view confocal endomicroscopy, which enables cellular imaging of gastrointestinal and respiratory tracts in mice
CO2 is the main inorganic C species entering photosynthetically active leaf protoplasts of the freshwater macrophyte Ranunculus penicillatus ssp. pseudofluitans.
Plant, Cell and Environment, 22, 1019-1026 (1999)
Brian P Eliceiri et al.
Methods in molecular biology (Clifton, N.J.), 686, 371-378 (2010-11-18)
The blood-brain barrier (BBB) is a monolayer of endothelial cells that is regulated by the proximity of a unique basement membrane and a tightly controlled molecular interaction between specialized subsets of cells including pericytes, astrocytes, and neurons. Working together, these
Yulian Zhou et al.
The Journal of clinical investigation, 124(9), 3825-3846 (2014-08-02)
Canonical WNT signaling is required for proper vascularization of the CNS during embryonic development. Here, we used mice with targeted mutations in genes encoding canonical WNT pathway members to evaluate the exact contribution of these components in CNS vascular development
Peterson RL, et al.
Teaching Plant Anatomy Through Creative Laboratory Exercises (2008)

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