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T2327

Sigma-Aldrich

Trypsin Inhibitor from Glycine max (soybean)

BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

Synonym(s):
Kunitz Inhibitor
CAS Number:
EC Number:
MDL number:
NACRES:
NA.77

biological source

Glycine max (soybean)

Quality Level

product line

BioUltra

assay

≥95% (Kunitz inhibitor, SDS-PAGE)

form

lyophilized powder

storage temp.

2-8°C

General description

Trypsin Inhibitor from Glycine max (soybean) also known as Kunitz trypsin inhibitor is a 21 kDa protein with a single trypsin binding reactive site.[34]

Application

Trypsin Inhibitor from Glycine max (soybean) has been used:
  • as a standard protein to measure the amount of endogenous trypsin inhibitor present in midgut lysate (M1) of Riptortus pedestris
[32]
  • as a standard to compare the trypsin inhibitory activity of the purified protein
  • [33]
  • to monitor the trypsin inhibitory activity by fractionating in MonoS cation exchange chromatography
  • [34]
  • as an trypsin inhibitor
  • [35]

    Packaging

    5, 10, 25 mg in glass bottle

    Biochem/physiol Actions

    Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108.
    Trypsin Inhibitor from Glycine max (soybean) binds with the active site of trypsin enzyme, in a competitive inhibition manner.[36]

    Unit Definition

    One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

    Preparation Note

    Further purification of T9128 yielding an electrophoretically pure Kunitz inhibitor with increased activity.
    Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.

    Analysis Note

    One mg will inhibit ≥1.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

    Other Notes

    View more information on Trypsin Inhibitor.

    Pictograms

    Health hazard

    Signal Word

    Danger

    Hazard Statements

    Hazard Classifications

    Resp. Sens. 1 - Skin Sens. 1

    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 3

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

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    Certificate of Origin

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