10109886001

Roche

Trypsin Inhibitor

from soybean

biological source

soybean

Quality Level

form

lyophilized

mol wt

Mr 20,100

packaging

pkg of 50 mg

manufacturer/tradename

Roche

concentration

1 mg/mL

application(s)

cell culture | mammalian: suitable

pH

3.8-4.5

solubility

water: soluble

shipped in

wet ice

storage temp.

2-8°C

General description

Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.

Specificity

Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).

Application

Trypsin Inhibitor has been used:
  • in the buffer for hepatocyte isolation
  • to inhibit trypsin in Corneal epithelial cells
  • to inhibit papain activity while treating hippocampal neural stem cells (NSCs)

Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.

Biochem/physiol Actions

Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.

Specifications

Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)

Isoelectric point: 4.5

Unit Definition

1 U = 270 S & T-units or 90 USP-units

Preparation Note

Working concentration: 10 to 100 μg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.

Reconstitution

Sensitive to heat, alkaline pH, and protein-precipitating compounds.

Storage and Stability

Store at 2 to 8 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Effects of soybean trypsin inhibitor on hemostasis
Soybean-Bio-Active Compounds (2013)
proBDNF is modified by advanced glycation end products in Alzheimer?s disease and causes neuronal apoptosis by inducing p75 neurotrophin receptor processing
Fleitas C, et al.
Molecular Brain, 11(1), 68-68 (2018)
Preparation of Primary Rat Hepatocyte Spheroids Utilizing the Liquid-Overlay Technique
Kyffin JA, et al.
Current Protocols in Toxicology / Editorial Board, Mahin D. Maines (editor-in-chief) ... [Et al.], 81(1), e87-e87 (2019)
Mice, double deficient in lysosomal serine carboxypeptidases Scpep1 and Cathepsin A develop the hyperproliferative vesicular corneal dystrophy and hypertrophic skin thickenings
Pan X, et al.
PLoS ONE, 12(2), e0172854-e0172854 (2017)
Isolating Taste Buds and Taste Cells from Vallate Papillae of C57BL/6J Mice for Detecting Transmitter Secretion
Anthony Y. Huang and Sandy Y. Wu
CorpBase ID (for auto-filling citation data), 6(11) (2016)

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