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T6414

Sigma-Aldrich

Trypsin inhibitor from Glycine max (soybean)

solution, sterile-filtered, suitable for, suitable for cell culture

Synonym(s):
SBTI
CAS Number:
MDL number:
NACRES:
NA.75

Quality Level

biological source

Glycine max (soybean)

sterility

sterile-filtered

product line

BioReagent

form

solution

mol wt

20,100 Da

concentration

10 mg/mL

application(s)

cell culture | mammalian: suitable

solubility

balanced salt solution: 1 mg/mL
concentrate: >10 mg/mL, hazy, amber-yellow
phosphate buffer: 10 mg/mL
water: 10 mg/mL
serum-free medium: soluble

suitability

suitable for

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

Glycine max (soybean) ... KTI3(547831)

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Application

Optimized for passage of endothelial cell cultures.
Trypsin inhibitor from Glycine max (soybean) was used for the following applications:
  • Filteration and seeding of dispersed acini
  • Relative quantification of phospho-tau and immunoblotting analysis
  • Subcloning and transformation of VIL-4 and IL-4 cDNA, and protein expression and purification (trypsin inhibitor form glycine max is used as positive control).

Biochem/physiol Actions

This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.

Other Notes

Contains chromatographically purified soybean trypsin inhibitor and iron supplemented calf serum.
View more information on Trypsin Inhibitor.

Unit Definition

One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.

Preparation Note

The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.

Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Prepared in Dulbecco′s Phosphate Buffered Saline without calcium and magnesium.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

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  4. What is the Department of Transportation shipping information for this product?

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Johann Gout et al.
Journal of visualized experiments : JoVE, (78)(78), doi:10-doi:10 (2013-08-28)
This protocol permits rapid isolation (in less than 1 hr) of murine pancreatic acini, making it possible to maintain them in culture for more than one week. More than 20 x 10(6) acinar cells can be obtained from a single...
Doubly Phosphorylated Peptide Vaccines to Protect Transgenic P301S Mice against Alzheimer's Disease Like Tau Aggregation.
Monique Richter et al.
Vaccine, 2(3), 601-623 (2014)
ZhuQing Yuan et al.
Journal of immunology (Baltimore, Md. : 1950), 182(2), 811-819 (2009-01-07)
The possibility that mycobacterial infections induce variant cytokine mRNA encoding a functionally distinct protein for immune regulation has not been addressed. In this study, we reported that Mycobacterium tuberculosis and bacillus Calmette-Guérin infections of macaques induced expression of variant IL-4...
Deepti Ramachandran et al.
Scientific reports, 8(1), 10818-10818 (2018-07-19)
Studies indicate that modulating enterocyte metabolism might affect whole body glucose homeostasis and the development of diet-induced obesity (DIO). We tested whether enhancing enterocyte fatty acid oxidation (FAO) could protect mice from DIO and impaired glycemic control. To this end...
Renpeng Guo et al.
Nature communications, 9(1), 2620-2620 (2018-07-07)
Feeder cells like mouse embryonic fibroblasts (MEFs) have been widely applied for culture of pluripotent stem cells, but their roles remain elusive. Noticeably, ESCs cultured on the feeders display transcriptional heterogeneity. We investigated roles of feeder cells by examining the...

Protocols

StableCell™ Trypsin Solutions

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

Human Astrocytes (HA) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Bovine Endometrial Endothelial Cells (BEnEpC) Culture Protocol

Technical information for working with bovine endometrial endothelial cells including thawing, subculturing and cryopreservation

Human Pulmonary Artery Endothelial Cells (HPAEC) Culture Protocol

Technical information for working with human pulmonary artery endothelial cells including thawing, subculturing and cryopreservation

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