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T0938

Sigma-Aldrich

Anti-Tumor Necrosis Factor-α antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym(s):

Anti-TNF-α

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About This Item

UNSPSC Code:
51111800
NACRES:
NA.41

biological source

goat

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

mouse

technique(s)

capture ELISA: suitable
flow cytometry: 5-25 μg/mL
indirect immunofluorescence: 15 μg/mL using mouse cells and tissues
neutralization: suitable
western blot: 0.1-0.2 μg/mL

UniProt accession no.

P06804

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Tnf(21926)

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General description

Tumor Necrosis Factor-α (TNF-α) is a protein produced by lipopolysaccharide-stimulated macrophages and causes tumor necrosis. It also facilitates many functions like stimulate growth of human fibroblasts and other cell lines, activate polymorphonuclear neutrophils and osteoclasts and collagenase production. Anti-Tumor Necrosis Factor-α antibody can be used for neutralizing the biological activity of recombinant mouse TNF-α. It can also be used in indirect immunofluorescence. Goat anti-Tumor Necrosis Factor-α antibody reacts specifically with recombinant mouse TNF-α.

Specificity

The antibody neutralizes the biological activity of recombinant mouse TNF-α.

Immunogen

recombinant mouse TNF-α

Application

Anti-Tumor Necrosis Factor-α antibody can be used in ELISA capture and flow cytometry. It can also be used in immunoblotting.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Preparation Note

Purified using mouse TNF-α affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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E A Carswell et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(9), 3666-3670 (1975-09-01)
In studying "hemorrhagic necrosis" of tumors produced by endotoxin, it was found that the serum of bacillus Calmette--Guerin (BCG)-infected mice treated with endotoxin contains a substance (tumor necrosis factor; TNF) which mimics the tumor necrotic action of endotoxin itself. TNF-positive
D R Bertolini et al.
Nature, 319(6053), 516-518 (1986-02-06)
When leukocytes are exposed to mitogens or antigens in vitro, they release bone-resorbing activity into the culture supernatants which can be detected by bioassay. Like many lymphocyte-monocyte products, this activity has been difficult to purify because of its low abundance
J M Dayer et al.
The Journal of experimental medicine, 162(6), 2163-2168 (1985-12-01)
Cachectin/TNF (tumor necrosis factor), an endotoxin-induced murine macrophage hormone implicated in the pathogenesis of cachexia and shock, has been found capable of stimulating collagenase and prostaglandin E2 (PGE2) production by isolated human synovial cells and dermal fibroblasts. This bioactivity associated
B J Sugarman et al.
Science (New York, N.Y.), 230(4728), 943-945 (1985-11-22)
Modulation of the growth of human and murine cell lines in vitro by recombinant human tumor necrosis factor-alpha (rTNF-alpha) and recombinant human interferon-gamma (rIFN-gamma) was investigated. rTNF-alpha had cytostatic or cytolytic effects on only some tumor cell lines. When administered
Jianhua Liu et al.
Frontiers in physiology, 8, 611-611 (2017-10-11)
Objective: This study aimed to investigate the effect of osmotin on myocardial ischemia/reperfusion (I/R), as well as the underlying mechanisms. Methods:In vitro I/R injury model was established on rat cardiac myoblast H9c2 cells by oxygen and glucose deprivation followed by
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