RAB0477

Sigma-Aldrich

Mouse Tumor Necrosis Factor α ELISA Kit

for serum, plasma and cell culture supernatant

Synonym(s):
TNF-alpha
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

application(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 60 pg/mL
standard curve range: 93.75-6000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Tnf(21926)

General description

The Mouse TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF-α in serum, plasma and cell culture supernatants.
Tumor Necrosis Factor α (TNF-α) is a proinflammatory cytokine. It is a transmembrane protein and the final protein is devoid of the extracellular domain. TNF-α-converting enzyme (TACE) catalyzes the formation of the soluble homotrimer TNF-α.

Immunogen

Recombinant Mouse TNF-α

Application

Mouse Tumor Necrosis Factor α ELISA Kit has been used in measuring TNF-αin MC3T3-E1 murine preosteoblastic calvarial cell lines, rat serum, mice liver and kidney samples.

Biochem/physiol Actions

Tumor Necrosis Factor α (TNF-α) elicits inflammatory and immuno-regulatory functionality. It mediates its effects via its receptors TNFR1 and TNFR2.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

hazcat

Met. Corr. 1

storage_class_code

8B - Non-combustible, corrosive hazardous materials

Certificate of Analysis

Certificate of Origin

Tumor necrosis factor alpha: a link between neuroinflammation and excitotoxicity
Olmos G and Llado J
Mediators of Inflammation (2014)
Curculigoside exerts significant anti-arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF-kappaB signaling pathway
Tan S, et al.
Molecular Medicine Reports, 19(3), 2057-2064 (2019)
Pharmacokinetic and pharmacodynamic characterisation of an anti-mouse TNF receptor 1 domain antibody formatted for in vivo half-life extension
Goodall LJ, et al.
PLoS ONE, 10(9), e0137065-e0137065 (2015)
Mangiferin inhibits apoptosis and oxidative stress via BMP2/Smad-1 signaling in dexamethasone-induced MC3T3-E1 cells
Ding LZ, et al.
International Journal of Molecular Medicine, 41(5), 2517-2526 (2018)
Zhaogang Yang et al.
Theranostics, 9(21), 6269-6283 (2019-09-20)
Sepsis is a major cause of patient mortality and morbidity from bacterial infections. Although neutrophils are known to be important in the development of sepsis, how distinctive neutrophil subtypes regulate inflammatory processes involved in septicemia remains unclear. Preconditioning protects organisms...

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