MilliporeSigma
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L4391

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O111:B4

γ-irradiated, BioXtra, suitable for cell culture

All Photos(2)
Synonym(s):
LPS
EC Number:
297-473-0
MDL number:
MFCD00164401
NACRES:
NA.75

Quality Level

300

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

purified by

gel-filtration chromatography

storage condition

desiccated

technique(s)

cell culture | mammalian: suitable

impurities

<1% Protein (Lowry)

solubility

H2O: 5 mg/mL, slightly hazy

storage temp.

2-8°C

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Lipopolysaccharides from Escherichia coli O111:B4 &#947;-irradiated, BioXtra, suitable for cell culture

Sigma-Aldrich

L4391

Lipopolysaccharides from Escherichia coli O111:B4

Lipopolysaccharides from Escherichia coli O111:B4 purified by gel-filtration chromatography

Sigma-Aldrich

L3012

Lipopolysaccharides from Escherichia coli O111:B4

Lipopolysaccharides from Escherichia coli O55:B5 &#947;-irradiated, BioXtra, suitable for cell culture

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L6529

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O26:B6 &#947;-irradiated, BioXtra, suitable for cell culture

Sigma-Aldrich

L2654

Lipopolysaccharides from Escherichia coli O26:B6

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 5 mg/mL, slightly hazy

solubility

-

solubility

H2O: 5 mg/mL, faintly hazy to hazy

solubility

H2O: 5 mg/mL, slightly hazy

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300

sterility

γ-irradiated

sterility

-

sterility

γ-irradiated

sterility

γ-irradiated

Application

This LPS serotype has been used to stimulate B-cells and induce NOS in human hepatocytes.
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans.

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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