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SAE0091

Sigma-Aldrich

Lysostaphin from Staphylococcus staphylolyticus

free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein

Synonym(s):
Glycyl-glycine Endopeptidase, Lysostaphin from Staphylococcus staphylolyticus
CAS Number:
Enzyme Commission number:
MDL number:

biological source

microbial (Staphylococcus staphylotyticus)

form

lyophilized powder

specific activity

≥500 units/mg protein

mol wt

27 kDa

feature

DNA free

shipped in

wet ice

storage temp.

−20°C

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Application

Purified extracellular lysostaphin from S. staphylolyticus SAE0091 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)

Biochem/physiol Actions

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5.
Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Unit Definition

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Physical form

Supplied as a lyophilized powder containing 50–70% protein with the balance primarily as NaCl.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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