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MAC4L

Sigma-Aldrich

MetaPolyzyme

lyophilized powder

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Synonym(s):
Multilytic Enzyme Mix

form

lyophilized powder

Quality Level

200

shipped in

wet ice

storage temp.

−20°C

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SAE0200MAC4LDFV2010
MetaPolyzyme lyophilized powder

Sigma-Aldrich

MAC4L

MetaPolyzyme

MycoPolyzyme for use with yeast and fungal lysis, free of DNA contaminants, suitable for Microbiome research

Sigma-Aldrich

SAE0200

MycoPolyzyme

MetaPolyzyme, DNA free Suitable for Microbiome research, lyophilized powder

Sigma-Aldrich

MAC4LDF

MetaPolyzyme, DNA free

Sigma-Aldrich

Sigma-Aldrich

V2010

Viscozyme® L

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

liquid

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Metagenomics analysis looks at all DNA that has been isolated directly from given single samples (e.g. environmental samples, biological organisms). Metagenomics allows for the investigation of microbes that exist in any environment (including extreme environments), and which have been historically difficult to isolate, culture, and study. Metagenomics has revealed the existence of novel microbial species. Applications of metagenomic studies include public health data analysis, discovery of novel proteins, enzymes and natural products, environmental studies, and agricultural investigations.

Application

MetaPolyzyme is a mixture of 6 enzymes that is intended for isolation of total DNA for metagenomics studies. Adapted from an initial formulation devised by S. Tighe,2 MetaPolyzyme was evaluated and developed in consultation and collaboration with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Research Group (MMRG). Test data on the use of MetaPolyzyme is accessible at the 2017 ABRF MGRG Poster.

Biochem/physiol Actions

Microbes can be difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted by using lysing enzymes (such as lyticase or chitinase) to induce partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. MetaPolyzyme is intended for use in metagenomic studies, such as study of microbes and microbiomes of extreme and unique environments. MetaPolyzyme is intended for digestion of microbes for evaluation by whole genome shotgun sequencing for metagenomics and metatranscriptomic approaches.

Components

The enzymes in MetaPolyzme are:
  • Achromopeptidase
  • Chitinase
  • Lyticase
  • Lysostaphin
  • Lysozyme
  • Mutanolysin

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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