MilliporeSigma
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S6147

Sigma-Aldrich

Sephadex® G-100

BioReagent, for molecular biology, DNA grade, medium

CAS Number:
MDL number:
NACRES:
NA.52

Quality Level

grade

DNA grade
for molecular biology

product line

BioReagent

form

beads

swelling

1 g swells to 9-11 mL

bead diameter

40-120 μm

pore size

exclusion limit (25 bp dsDNA; (rA)27)

pH

2-10

foreign activity

DNase, RNase, none detected

storage temp.

room temp

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General description

Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Application

Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.

Packaging

25, 100 g in poly bottle

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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