MilliporeSigma
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S5772

Sigma-Aldrich

Sephadex® G-25

BioReagent, for molecular biology, DNA grade, Superfine

CAS Number:
MDL number:

Quality Level

grade

DNA grade
for molecular biology

product line

BioReagent

form

beads

bead diameter

20-50 μm

pore size

exclusion limit (10 bp dsDNA; (rA)10)

pH range

2-13

foreign activity

DNase, RNase, none detected

storage temp.

room temp

InChI

1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1

InChI key

BCPVZFFJGCURNR-OCOFDJSDSA-N

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General description

Sephadex® G-25, Superfine is an often-cited gel filtration medium for desalting and buffer exchange. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Superfine” indicates the smallest particle size available.

Application

Sephadex® G-25 is suitable for buffer exchange and desalting oligonucleotides for use in sequencing, PCR, and labeling applications. It was used to isolate two different peptides of Pronase E extracted from Alaska Pollack skin.

Packaging

10, 25, 100 g in poly bottle

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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