MilliporeSigma
  • S5772
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S5772

Sigma-Aldrich

Sephadex® G-25

BioReagent, for molecular biology, DNA grade, Superfine

CAS Number:
MDL number:

grade

DNA grade
for molecular biology

Quality Level

product line

BioReagent

form

beads

bead diameter

20-50 μm

pore size

exclusion limit (10 bp dsDNA; (rA)10)

pH range

2-13

foreign activity

DNase, RNase, none detected

storage temp.

room temp

InChI

1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1

InChI key

BCPVZFFJGCURNR-OCOFDJSDSA-N

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Sephadex® G-25 BioReagent, for molecular biology, DNA grade, Superfine

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Sephadex® G-50 BioReagent, for molecular biology, DNA grade, fine

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Sephadex® G-50

grade

DNA grade

grade

DNA grade, for molecular biology

grade

DNA grade, for molecular biology

grade

DNA grade, for molecular biology

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

BioReagent

form

beads

form

beads

form

beads

form

beads

bead diameter

20-50 μm

bead diameter

40-120 μm

bead diameter

50-150 μm

bead diameter

20-80 μm

pore size

exclusion limit (10 bp dsDNA; (rA)10)

pore size

exclusion limit (25 bp dsDNA; (rA)27)

pore size

exclusion limit (20 bp dsDNA; (rA)20)

pore size

exclusion limit (20 bp dsDNA (rA)20)

pH range

2-13

pH range

-

pH range

-

pH range

-

General description

Sephadex® G-25, Superfine is an often-cited gel filtration medium for desalting and buffer exchange. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Superfine” indicates the smallest particle size available.

Application

Sephadex® G-25 is suitable for buffer exchange and desalting oligonucleotides for use in sequencing, PCR, and labeling applications. It was used to isolate two different peptides of Pronase E extracted from Alaska Pollack skin.

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

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