MilliporeSigma
All Photos(2)

S6022

Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, medium

CAS Number:
MDL number:

Quality Level

grade

DNA grade
for molecular biology

product line

BioReagent

form

beads

swelling

1 g swells to 9-11 mL

bead diameter

50-150 μm

pore size

exclusion limit (20 bp dsDNA; (rA)20)

pH

2-10

foreign activity

DNase, RNase, none detected

storage temp.

room temp

Looking for similar products? Visit Product Comparison Guide

General description

Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Application

Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions.
Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.

Packaging

25, 100 g in poly bottle

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service