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Sephadex® G-25


CAS Number:
MDL number:

Quality Level


LPLC: suitable


1 g swells to 4-6 mL

bead size

20-80 μm

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InChI key


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General description

Sephadex® is a beaded gel filtration medium prepared by cross-linking dextran with epichlorohydrin under alkaline conditions. It is used in affinity chromatography, protein chromatography and gel filtration chromatography.
Fractionation Range (MW)
Globular Proteins: 1,000 - 5,000
Dextrans: 100 - 5,000


Sephadex® G-25 has been used in size-exclusion chromatography to remove excess dye from labelled proteins.


10, 50, 100, 500 g in poly bottle

Other Notes

G2580-100G′s updated product number is GE17-0032-01
G2580-500G′s updated product number is GE17-0032-02

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Celine Hoffmann et al.
Analytical biochemistry, 372(1), 62-71 (2007-09-11)
Fluorescein isothiocyanate (FITC) is a well-known probe for labeling biologically relevant proteins. However, the impact of the labeling procedure on protein structure and biological activities remains unclear. In this work, FITC-labeled human plasma fibronectin (Fn) was developed to gain insight...
Jennifer Belyea et al.
Biochemistry, 45(48), 14275-14284 (2006-11-30)
The study of axial ligation by anionic ligands to ferric heme iron by resonance Raman spectroscopy provides a basis for comparison of the intrinsic electron donor ability of the proximal histidine in horse heart myoglobin (HHMb), dehaloperoxidase (DHP), and horseradish...
Conformations of a metastable SH3 domain characterized by smFRET and an excluded-volume polymer model.
Mazouchi A, et al.
Biophysical Journal, 110(7), 1510-1522 (2016)
Jana Schwarzer et al.
Electrophoresis, 29(20), 4203-4214 (2008-10-18)
Glycoproteins, such as monoclonal antibodies as well as recombinant and viral proteins produced in mammalian cell culture play an important role in manufacturing of many biopharmaceuticals. To ensure consisting quality of the corresponding products, glycosylation profiles have to be tightly...
P M G Paiva et al.
Bioresource technology, 88(1), 75-79 (2003-02-08)
A highly purified trypsin inhibitor was obtained from Echinodorus paniculatus when an extract prepared from E. paniculatus seed flour (25 gl(-1), with 0.1 M ammonium acetate buffer, pH 8.3, under agitation for 6 min at 28 degrees C) was chromatographed...

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