slurry
LPLC: suitable
Agarose
polymer
1 g swells to 4 mL (water, methanol)
25-100 μm
2-13
size exclusion (SEC)
room temp
13 - Non Combustible Solids
WGK 3
Not applicable
Not applicable
dust mask type N95 (US), Eyeshields, Gloves
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Sephadex LH-20 is a beaded, cross-linked dextran which has been hydroxypropylated to yield a chromatographic media with both hydrophilic and lipophilic character. Due to its dual character, LH-20 swell in water and a number of organic solvents. The particle size and exclusion limit vary depending on the solvent used for swelling. The swollen resin can be used for gel filtration separations of both hydrophilic and lipophilic compounds.
Sephadex LH-20 swells in water, polar organic solvents, aqueous solvent mixtures, and mixtures of polar organic solvents. In solvent mixtures containing both polar and non-polar organic solvents the gel takes up predominantly the polar component. Following is a table of solvents and the approximate swelling volume. Solvents giving a bed volume of less than 2.5 ml/g dry gel are generally not used.Solvent Approx. bed volume (ml/g dry gel)Dimethyl sulfoxide 4.4 - 4.6Pyridine 4.2 - 4.4Water 4.0 - 4.4Dimethyl formamide 4.0 - 4.4Methanol 3.9 - 4.3Chloroform 3.8 - 4.1Ethanol 3.6 - 3.9Methylene dichloride 3.6 - 3.9Tetrahydrofuran 3.3 - 3.6Acetone 2.4 - 2.6Acetonitrile 2.2 - 2.4Benzene 1.6 - 2.0Ethyl acetate 1.6 - 1.8Toluene 1.5 - 1.6
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