All Photos(1)



Sephadex® LH-20

Lipophilic Sephadex®

Quality Level




storage room temp.


LPLC: suitable



matrix active group



1 g swells to 4 mL (water, methanol)

bead size

25-100 μm

operating pH range

2 - 13

separation technique

size exclusion (SEC)

Related Categories


Sephadex® LH-20 is a crosslinked dextran-based resin used with organic solvent mobile phases for gel permeation, normal phase partition, and adsorption chromatography of lipids, steroids, fatty acids, hormones, vitamins, etc. It is also used in gel filtration chromatography, protein chromatography, gel filtration media, resins and separation media.
Sephadex® LH-20 has been used to isolate bioactive polyphenolic content from blackberry genotypes and tryptophan from the aqueous extract of lotus rhizomes.


50, 100, 6×100, 500 g in poly bottle

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is Sephadex LH-20, Product LH20100?

    Sephadex LH-20 is a beaded, cross-linked dextran which has been hydroxypropylated to yield a chromatographic media with both hydrophilic and lipophilic character.  Due to its dual character, LH-20 swell in water and a number of organic solvents.  The particle size and exclusion limit vary depending on the solvent used for swelling.  The swollen resin can be used for gel filtration separations of both hydrophilic and lipophilic compounds.

  4. What solvents can be used with Sephadex LH-20, Product LH20100?

    Sephadex LH-20 swells in water, polar organic solvents, aqueous solvent mixtures, and mixtures of polar organic solvents.  In solvent mixtures containing both polar and non-polar organic solvents the gel takes up predominantly the polar component.   Following is a table of solvents and the approximate swelling volume.  Solvents giving a bed volume of less than 2.5 ml/g dry gel are generally not used.Solvent                           Approx. bed volume (ml/g dry gel)Dimethyl sulfoxide           4.4 - 4.6Pyridine                          4.2 - 4.4Water                             4.0 - 4.4Dimethyl formamide        4.0 - 4.4Methanol                        3.9 - 4.3Chloroform                     3.8 - 4.1Ethanol                           3.6 - 3.9Methylene dichloride       3.6 - 3.9Tetrahydrofuran              3.3 - 3.6Acetone                          2.4 - 2.6Acetonitrile                     2.2 - 2.4Benzene                         1.6 - 2.0Ethyl acetate                   1.6 - 1.8Toluene                          1.5 - 1.6

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

A K Tandon et al.
Cancer research, 46(7), 3375-3377 (1986-07-01)
We described recently a modified charcoal-gelatin (MCG) assay for measuring progesterone receptor activity in low-protein cytosols. We showed that pre-mixing of gelatin with sample cytosols (final gelatin concentration 0.1%) and removal of unbound steroid by a 1% charcoal suspension with...
A Cahouet et al.
International journal of pharmaceutics, 242(1-2), 367-371 (2002-08-15)
The aim of the present work was to study the biodistribution of a radiolabeled lipidic nanocapsule formulation after intravenous administration in rat by scintigraphy and gamma counting. This formulation is expected to be used as anticancer agent delivery devices and...
Y Jiang et al.
International journal of food sciences and nutrition, 61(4), 346-356 (2010-05-15)
Various vegetables were investigated for antioxidant activities in two assays, namely, inhibition of lysis of erythrocytes induced by peroxyl radicals and inhibition of lipid peroxidation. The lotus (Nelumbo nucifera Gaertn) rhizome showed the strongest antioxidant activity in both assays. The...
Attila Hunyadi et al.
PloS one, 6(8), e23922-e23922 (2011-09-14)
Protoapigenone, a natural flavonoid possessing an unusual p-quinol moiety on its B-ring, is a novel prospective anticancer agent with low toxicity that is currently in development. The first economical, one-step synthesis of protoapigenone from apigenin is described on up to...
Z Piskornik et al.
Plant physiology, 50(1), 176-182 (1972-07-01)
The "bound auxin" of Zea mays, first described by Berger and Avery (Amer. J. Bot. 1944; 31: 199-203) has been purified and partially characterized. It is an indole-3-acetic acid-containing, high molecular weight, lipophilic cellulosicglucan. The indole-3-acetic acid is in ester...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service