S5395

Sigma-Aldrich

Superoxide Dismutase from bovine erythrocytes

BioReagent, ≥3,000 units/mg protein, suitable for cell culture, lyophilized powder

Synonym(s):
Superoxide: superoxide oxidoreductase, SOD
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.75

Quality Level

biological source

bovine erythrocytes

product line

BioReagent

form

lyophilized powder

specific activity

≥3,000 units/mg protein

mol wt

32.5 kDa

packaging

pkg of 15000 units

application(s)

cell culture | mammalian: suitable

pH

7.6-10.5

shipped in

dry ice

storage temp.

−20°C

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General description

Superoxide Dismutase from bovine erythrocytes is a metalloprotein which disproportionates superoxide anion radicals. It is a 31.5 kDa copper binding protein and displays a conserved domain and fold. It is a homodimer with one copper and zinc ion per subunit and has antiparallel “greek-key” β barrel fold.

Application

Superoxide Dismutase (SOD) from bovine erythrocytes has been used:
  • for measuring the superoxide radical using the electron paramagnetic resonance spin in human brain microvascular endothelial cells
  • for measuring superoxide production in cytochrome C assay in peripheral blood mononuclear cells
  • as a standard in characterization of hen egg SOD using Fourier-transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption/ionization (MALDI) analysis

Packaging

30000, 75000 units in poly bottle
15000 units in glass bottle

Biochem/physiol Actions

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Unit Definition

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.

Analysis Note

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Zoe Loomis et al.
PloS one, 12(2), e0171219-e0171219 (2017-02-06)
It is now well established that both inherited and acquired forms of hemolytic disease can promote pulmonary vascular disease consequent of free hemoglobin (Hb) induced NO scavenging, elevations in reactive oxygen species and lipid peroxidation. It has recently been reported...
Chunxia Xiao et al.
PloS one, 7(1), e30343-e30343 (2012-01-25)
The C57BLKS/J db/db (db/db) mouse is a widely used type 2 diabetic animal model, and this model develops early inner retinal neuronal dysfunction beginning at 24 weeks. The neural mechanisms that mediate early stage retinal dysfunction in this model are...
Partial biochemical characterization of Cu, Zn-superoxide dismutase extracted from eggs of hens (Gallus gallus domesticus)
Wawrzykowski J and Kankofer M
Food Chemistry, 227, 390-396 (2017)
Lipotoxic brain microvascular injury is mediated by activating transcription factor 3-dependent inflammatory and oxidative stress pathways
Aung HH, et al.
Journal of Lipid Research, 57(6), 955-968 (2016)
The structural biochemistry of the superoxide dismutases
Perry JJP, et al.
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1804(2), 245-262 (2010)

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