C1345

Catalase from bovine liver

powder, suitable for cell culture, 2,000-5,000 units/mg protein

• Synonym(s): H₂O₂:H₂O₂ oxidoreductase
• CAS Number: 9001-05-2
• Enzyme Commission number: 1.11.1.6 (BRENDA, IUBMB)
• EC Number: 232-577-1
• MDL number: MFCD00081483
• eCl@ss: 32160410
• NACRES: NA.75

Properties

• biological source: bovine liver
• Quality Level: 200
• product line: BioReagent
• form: powder
• specific activity: 2,000-5,000 units/mg protein
• mol wt: tetramer ~250 kDa
• technique(s): cell culture | mammalian: suitable
• isoelectric point: 5.4
• UniProt accession no.: P00432
• shipped in: wet ice
• storage temp.: −20°C
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
• InChI key: NUVBSKCKDOMJSU-UHFFFAOYSA-N
• Gene Information: cow ... CAT(280743)

Description

General description

CAT (catalase) is an endogenous antioxidant enzyme, thus conferring protection to cells against damage by ROS (reactive oxygen species). In humans, this gene is localized to chromosome 11p13, which is composed of 12 introns and 13 exons.

Application

Catalase from bovine liver is used for the following applications:

• Gel filtration
• Analytical filtration on Ultrogel and glycerol gradient centrifugation
• Size exclusion chromatography (HPLC)-HPLC analysis of estrogen receptor
• Enzyme assays
• Alcohol oxidase activity

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Biochem/physiol Actions

Catalase catalyzes the degradation of hydrogen peroxide into water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H₂O₂ molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor.

This product doesn not need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

Components

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Caution

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Unit Definition

One unit will decompose 1.0 μmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

Preparation Note

This product is a powder suitable for cell culture with activity at 2,000-5,000 units/mg. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves