MilliporeSigma
All Photos(4)

T0665

Sigma-Aldrich

holo-Transferrin human

powder, BioReagent, suitable for cell culture, ≥97%

All Photos(4)
Synonym(s):
Siderophilin, iron-saturated
CAS Number:
11096-37-0
EC Number:
234-318-8
MDL number:
MFCD00131337
NACRES:
NA.75

Quality Level

200

biological source

human plasma

description

Iron-saturated

product line

BioReagent

assay

≥97%

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

HIV and HBsAg, none detected
 endotoxin, tested

solubility

H2O: 50 mg/mL

UniProt accession no.

P02787

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... TF(7018)

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Application

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.
holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy.

Packaging

50, 100, 500 mg in poly bottle
1 g in poly bottle

Biochem/physiol Actions

Provides iron to cells in a physiologically stable and safe form.

Preparation Note

Heat treated at 60 °C (minimum) for a minimum of 10 hours.

Analysis Note

Purity by agarose gel electrophoresis.
Iron content by ICP reported on a lot-specific basis.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the carbohydrate content of human transferrin?

    We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.

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