MilliporeSigma
All Photos(5)

T1147

Sigma-Aldrich

apo-Transferrin human

powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)

Synonym(s):
Human transferrin, Siderophilin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

Quality Level

biological source

human plasma

product line

BioReagent

assay

≥98% (agarose gel electrophoresis)

form

powder

mol wt

76-81 kDa

concentration

~25 mM

technique(s)

cell culture | mammalian: suitable

impurities

HIV and HBsAg, source material tested negative
≤0.005% iron

solubility

H2O: 50 mg/mL

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TF(7018)

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Application

Apo-Transferrin human can be supplemented with iron or used to bind free iron present in media. It is also used to transport iron into mammalian cells.

Packaging

100, 5×100, 500 mg in poly bottle
1, 5 g in poly bottle

Biochem/physiol Actions

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human apo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Apo-transferrin can be loaded with iron prior to use or added directly to an iron containing medium.

Other Notes

Non-heme iron-transport protein.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the carbohydrate content of human transferrin?

    We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.

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