All Photos(3)

NA2100

Sigma-Aldrich

GenElute Bacterial Genomic DNA Kits

sufficient for 10 purifications

Synonym(s):
Bacterial Genomic DNA, Gen Elute
NACRES:
NA.55

usage

sufficient for 10 purifications

Quality Level

storage temp.

15-25°C

Related Categories

General description

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified bacterial genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • cloning

Features and Benefits

  • Starting material: Up to 1.5 mL of culture
  • Expected yield: Up to 20 μg
  • Elution volume: 400 μl
  • Time required: 70 - 120 min
  • A260/A280 ratio: 1.6 - 1.9
  • No phenol, chloroform, or ethanol precipitation required

Principle

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • W0263Wash Solution 1

  • C2112Column Preparation Solution

  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

  • R6148RNase A solution

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Certificate of Analysis

Certificate of Origin

K E Eboigbodin et al.
Applied microbiology and biotechnology, 73(3), 669-675 (2006-07-21)
The hypothesis tested in this paper is that quorum sensing influences the microbial surface electrokinetic properties. Escherichia coli MG1655 and MG1655 LuxS- mutant (lacking quorum-sensing gene for Autoinducer synthase AI-2) were used for this study. AI-2 production (or lack of)
Tracy H Hazen et al.
Journal of bacteriology, 194(11), 3028-3029 (2012-05-15)
Klebsiella oxytoca strain 11492-1 was isolated from a perianal swab culture from a patient at the University of Maryland Medical Center in 2005. The K. oxytoca 11492-1 draft genome contains multiple antibiotic resistance genes, including a FOX-5 AmpC β-lactamase encoded
Fean D Sarian et al.
Applied microbiology and biotechnology, 93(2), 645-654 (2011-07-07)
Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium
Seyed Mohammad Mehdi Dastgheib et al.
Applied microbiology and biotechnology, 95(3), 789-798 (2011-11-17)
In this study we investigated the phenanthrene degradation by a halophilic consortium obtained from a saline soil sample. This consortium, named Qphe, could efficiently utilize phenanthrene in a wide range of NaCl concentrations, from 1% to 17% (w/v). Since none
Chityal Ganesh Kumar et al.
Biotechnology progress, 28(6), 1507-1516 (2012-09-11)
Eleven biosurfactant producing bacteria were isolated from different petroleum-contaminated soil and sludge samples. Among these 11 isolates, two were identified as promising, as they reduced the surface tension of culture medium to values below 27 mN m(-1) . Besides biosurfactant

Protocols

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service