All Photos(3)

11814770001

Roche

DNA Isolation Kit for Cells and Tissues

sufficient for 10 isolation(s)

usage

sufficient for 10 isolation(s)

Quality Level

manufacturer/tradename

Roche

packaging

kit of for 10 isolations

General description

The DNA Isolation Kit for Cells and Tissues provides medium- and large-scale preparation of purified genomic DNA ranging in size from 50 to 150 kb. Remove contaminating RNA and proteins from a wide variety of biological specimens (mammalian tissue, cultured cells, yeast, gram-negative bacteria or mouse tail).

Application

DNA Isolation Kit for Cells and Tissues has been used to isolate DNA from a wide variety of starting materials. The isolated DNA is suitable for many molecular biology applications:
  • Genomic Southern blotting
  • Sequencing
  • Restriction digestion
  • PCR/long PCR
  • Cloning

Features and Benefits

Obtain increased yields of DNA in less than 2.5 hours.
Benefit from a simple, straightforward procedure (compared with column based methods).
Increase safety and convenience.
Eliminate the use of chaotropic salts, anion-exchange columns, and hazardous organic solvents.
Reduce purification time.
All required reagents are included in the kit.

Components

  • Cellular Lysis Buffer
  • Proteinase K Solution
  • RNase Solution
  • Protein Precipitation Solution

Quality

Each lot of the DNA Isolation Kit for Cells and Tissues is tested for the absence of DNase contamination. Function tests to purify DNA from bovine liver, followed by specific amplification with the Expand High Fidelity PCR System were performed.

Preparation Note

Sample material is homogenized in Cellular Lysis Buffer in the presence of a strong anionic detergent and proteinase K. RNA is eliminated by RNase treatment and proteins are removed by selective precipitation and centrifugation. The purified DNA is finally recovered from solution by isopropanol precipitation.

Other Notes

Figure 1: Amplification of both short and long DNA fragments from genomic DNA prepared with the DNA Isolation Kit for Cells and Tissues. Genomic DNA was isolated from a variety of sources and then amplified with either Taq DNA Polymerase, the Expand High Fidelity PCR System, or the Expand Long Template PCR System.
Lanes 2, 3: Human DMD fragment (268 bp) and mouse c-myc fragment (580 bp), amplified with Taq DNA Polymerase
Lanes 4, 5, 7, 8: Human c-myc fragment (1.2 kb), mouse β2-microglobulin fragment (3.6 kb), bovine lysozyme gene fragment (6.9 kb), and human tPA gene fragment (9.3 kb), all amplified with the Expand High Fidelity PCR System
Lanes 6, 9, 10: Mouse α-2 collagen gene fragments (5.6 kb and 10.4 kb) and human β-globin fragment (23 kb), amplified with the Expand Long Template PCR System
Lanes 1, 11 : DNA Molecular Weight Markers VI and II
For life science research only. Not for use in diagnostic procedures.
Purity of isolated DNA: Average A 260 / A 280 ratio: 1.7 - 1.9

Isolation of High Molecular Weight DNA
The kit simplifies the isolation of 50 to 150 kb genomic DNA.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Shahrzad Atapoor et al.
Jundishapur journal of microbiology, 7(5), e10013-e10013 (2014-08-26)
There is a possibility for the presence of Helicobacter pylori in vegetables due to their close contact with polluted water, soil and feces. This study was carried out to detect the presence of H. pylori in vegetables and salads in
The first case of NSHL by direct impression on EYA1 gene and identification of one novel mutation in MYO7A in the Iranian families.
Ehsan R, et al.
Iranian Journal of Basic Medical Sciences, 21(3), 333- 341 (2018)
Fatemeh Bitarafan et al.
Molecular and cellular pediatrics, 8(1), 6-6 (2021-05-11)
Ca2+ as a universal second messenger regulates basic biological functions including cell cycle, cell proliferation, cell differentiation, and cell death. Lack of the protein mitochondrial calcium uptake1 (MICU1), which has been regarded as a gatekeeper of Ca ions, leads to
VacA, cagA, iceA and oipA genotypes status and antimicrobial resistance properties of Helicobacter pylori isolated from various types of ready to eat foods
Hemmatinezhad B, et al.
Annals of Clinical Microbiology and Antimicrobials, 15(1), 2-2 (2016)

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